Expression of COX-2 and PCNA and correlation with the clinical characteristics of colorectal cancer

ZHANG Li-jing; et al

doi:10.13406/j.cnki.cyxb.2010.10.038

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Expression of COX-2 and PCNA and correlation with the clinical characteristics of colorectal cancer

更新时间：2024-01-17

- Expression of COX-2 and PCNA and correlation with the clinical characteristics of colorectal cancer
- Journal of Chongqing Medical University Vol. 35, Issue 10, Pages: 1467-1470(2010)
- 作者机构：
  
  河北医科大学第一医院普外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.10.038
  CLC： R735.3
- Published：2010，
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[1]张丽静,赵增仁,王园园,李芳,张志勇.COX-2和PCNA的表达与结直肠癌临床特征间的关系[J].重庆医科大学学报,2010,35(10):1467-1470.

ZHANG Li-jing, et al. Expression of COX-2 and PCNA and correlation with the clinical characteristics of colorectal cancer. [J]. Journal of Chongqing Medical University 35(10):1467-1470(2010)
[1]张丽静,赵增仁,王园园,李芳,张志勇.COX-2和PCNA的表达与结直肠癌临床特征间的关系[J].重庆医科大学学报,2010,35(10):1467-1470. DOI： 10.13406/j.cnki.cyxb.2010.10.038.

ZHANG Li-jing, et al. Expression of COX-2 and PCNA and correlation with the clinical characteristics of colorectal cancer. [J]. Journal of Chongqing Medical University 35(10):1467-1470(2010) DOI： 10.13406/j.cnki.cyxb.2010.10.038.

摘要

目的:检测结直肠癌组织中环氧合酶-2（Cyclooxygenase-2

COX-2）和增殖细胞核抗原（Proliferating cell nuclear antigen

PCNA）的表达

分析与肿瘤临床病理特征的关系;同时检测结直肠癌患者血浆中前列腺素E2（Prostaglandin E2

PGE2）的表达。方法:采用定量逆转录聚合酶链反应方法

检测40例结直肠癌手术标本中的肿瘤及癌旁无瘤结肠组织中COX-2、PCNAmRNA转录水平

并结合临床病理指标进行分析;同时采用酶联免疫吸附实验（ELISA）检测结直肠癌术前、术后及健康对照人群血清中PGE2的表达差异。结果:肿瘤组织中COX-2和PCNA的mRNA转录水平显著高于癌旁无瘤结肠组织（P<0.01）

肿瘤组织中COX-2的mRNA转录水平在不同的组织分化程度、浸润深度和有无淋巴结转移中具有统计学差异（P<0.05）;PCNA的mRNA转录水平与肿瘤组织的分化程度和浸润深度有关（P<0.05）

和淋巴结转移无关;且两者表达呈显著正相关（P<0.01）。结直肠癌患者术前及术后血浆中PGE2水平明显高于正常对照组（P<0.05）

而且术后血浆中PGE2的表达较术前显著下降（P<0.01）

但仍高于对照组（P<0.05）。结论:COX-2和PCNA在结直肠癌的恶性生物学行为中起重要作用

监测外周血中PGE2水平对早期发现结直肠癌有一定参考价值。

Abstract

Objective:To investigate the expression of cyclooxygenase-2 (COX-2)and proliferation cell nuclear antigen (PCNA)in colorectal carcinoma

and to identify the correlation between these expressions with the clinical biological features. Methods:The levels of COX-2 and PCNA mRNA were detected with quantitative reverse transcription-polymerase chain reaction (QRT-PCR)in 40 pairs of samples containing tumor tissues and the non-cancerous tissues. The level of PGE2 in peripheral blood was detected by ELISA. Data of COX-2 and PCNA mRNA were statistically analyzed along with the corresponding clinicopathological features. Results:The mRNA transcription levels of COX-2 and PCNA in tumor tissues were significantly higher than that in the non-cancerous tissues (P<0.01). A significant difference was found in mRNA transcription levels for COX-2 and PCNA among patients with tumors of different degrees of invasion depth and the differentiation. The mRNA transcription level of COX-2 was significantly different between patients with and without lymph node metastasis(P<0.05)

which was not found in PCNA. COX-2 mRNA expression was correlated with PCNA in colorectal cancer (P<0.01). The PGE2 levels in postoperative plasma of colorectal cancer was significantly lower than that in preoperative plasma (P<0.01)

but still higher than the normal plasma (P<0.05). Conclusion:COX-2 and PCNA play important roles in carcinogenesis of colorectal cancer . Measurement of PGE2 might be helpful in the early detection and diagnosis of colorectal cancer.

关键词

环氧合酶2前列腺素E2结直肠癌增殖细胞核抗原

Keywords

Cyclooxygenase-2prostaglandin E2colorectal carcinomaProliferation cell nuclear antigen

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