Effect of endoplasmic reticulum stress on apoptosis of cardiomyocyte in hyperlipidemia rats

ZHOU Ping; et al

doi:10.13406/j.cnki.cyxb.2010.10.021

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Effect of endoplasmic reticulum stress on apoptosis of cardiomyocyte in hyperlipidemia rats

更新时间：2024-01-17

- Effect of endoplasmic reticulum stress on apoptosis of cardiomyocyte in hyperlipidemia rats
- Journal of Chongqing Medical University Vol. 35, Issue 10, Pages: 1509-1512(2010)
- 作者机构：
  
  1. 重庆医科大学附属第一医院老年病科
  2. 重庆医科大学附属第一医院肾内科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.10.021
  CLC： R589.2
- Published：2010，
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[1]周平,张彬,翟福利,李法琦,常晓东,甘华.内质网应激在高脂血症大鼠心肌细胞凋亡中的作用[J].重庆医科大学学报,2010,35(10):1509-1512.

ZHOU Ping, et al. Effect of endoplasmic reticulum stress on apoptosis of cardiomyocyte in hyperlipidemia rats. [J]. Journal of Chongqing Medical University 35(10):1509-1512(2010)
[1]周平,张彬,翟福利,李法琦,常晓东,甘华.内质网应激在高脂血症大鼠心肌细胞凋亡中的作用[J].重庆医科大学学报,2010,35(10):1509-1512. DOI： 10.13406/j.cnki.cyxb.2010.10.021.

ZHOU Ping, et al. Effect of endoplasmic reticulum stress on apoptosis of cardiomyocyte in hyperlipidemia rats. [J]. Journal of Chongqing Medical University 35(10):1509-1512(2010) DOI： 10.13406/j.cnki.cyxb.2010.10.021.

摘要

目的:研究内质网应激是否在高脂血症大鼠诱导心肌细胞凋亡中起作用。方法:通过建立高脂血症大鼠模型

全自动化生化仪检测血清甘油三酯（Triglycercide

TG）及胆固醇（Cholesterol

TC）水平;HE染色观察心肌组织的病理学变化;TUNEL法检测心肌细胞凋亡;免疫组化法及RT-PCR技术检测心肌细胞内质网应激（Endoplasmic reticulum stress

ERS）信号通路分子GRP78的表达变化。结果:喂养12周时模型组大鼠血清TC、TG含量明显高于对照组（P<0.01）;大鼠模型组较正常组心肌组织排列紊乱、边界不清、心肌纤维断裂、部分细胞核溶解消失;模型组大鼠心肌细胞凋亡率明显高于正常组（P<0.05）;大鼠心肌GRP78mRNA及蛋白的表达量

模型组均较对照组明显升高（P<0.05）。结论:ERS途径可能在高脂血症诱导心肌细胞凋亡中起到重要作用。

Abstract

Objective:To investigate the mechanism of effects of endoplasmic reticulum stress on apoptosis of cardiomyocyte in hyperlipidemia rats. Methods:Rat model of established with fatty dieting.After twelve weaks

the serum triglycerides(TG)and cholesterol (TC)were oberserved by completely automatic biochemistry meter;The pathological changes of myocardial tissue were detected by HE staining;The apoptosis of cardiomyocyte were tested by TUNEL. The expression of endoplasmic reticulum stress associated molecular glucose regulated protein 78 in myocardial tissue were tested byimmunohistochemistry and RT-PCR. Results :The levels of TC and TG in blood sera of control group did not change after 12 weeks

while TC and TG in model group significantly increased along with the te mporal prolongation compared with those in control group ( P <0.01 ) . The expression of GRP78 mRNA and protein in myocardiocyte of rats in model group significantly increased with those in control group ( P<0.05 ) . Enhanced expression of GRP78 could be induced by fatty diet. Couclusion:The hyperlipidemia maybe can induce myocardiocyte apoptosis through endoplasmic reticulum pathways.

关键词

高脂血症内质网应激心肌细胞凋亡

Keywords

hyperlipidemiaendoplasmic reticulum stressCardiomycyteapoptosis

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