Action of uricase to lower plasma urate in hyperuricemic rat model developed with oxonate

GAN Zhi-yong; et al

doi:10.13406/j.cnki.cyxb.2010.10.019

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Action of uricase to lower plasma urate in hyperuricemic rat model developed with oxonate

更新时间：2024-01-17

- Action of uricase to lower plasma urate in hyperuricemic rat model developed with oxonate
- Journal of Chongqing Medical University Vol. 35, Issue 10, Pages: 1454-1457(2010)
- 作者机构：
  
  1. 重庆医科大学重庆市生物化学和分子药理学重点实验室
  2. 重庆医科大学附属儿童医院药剂科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.10.019
  CLC： R589.7
- Published：2010，
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[1]甘志勇,张纯,冯娟,杨晓兰,袁拥华,廖飞.尿酸酶在用氧嗪酸建立高尿酸血症模型大鼠体内的降尿酸作用[J].重庆医科大学学报,2010,35(10):1454-1457.

GAN Zhi-yong, et al. Action of uricase to lower plasma urate in hyperuricemic rat model developed with oxonate. [J]. Journal of Chongqing Medical University 35(10):1454-1457(2010)
[1]甘志勇,张纯,冯娟,杨晓兰,袁拥华,廖飞.尿酸酶在用氧嗪酸建立高尿酸血症模型大鼠体内的降尿酸作用[J].重庆医科大学学报,2010,35(10):1454-1457. DOI： 10.13406/j.cnki.cyxb.2010.10.019.

GAN Zhi-yong, et al. Action of uricase to lower plasma urate in hyperuricemic rat model developed with oxonate. [J]. Journal of Chongqing Medical University 35(10):1454-1457(2010) DOI： 10.13406/j.cnki.cyxb.2010.10.019.

摘要

目的:用尿酸酶抑制剂氧嗪酸建立高尿酸血症大鼠模型

考察尿酸酶的体内降尿酸作用。方法:大鼠腹腔注射氧嗪酸钾建立高尿酸血症大鼠模型

尿酸酶滤菌后尾静脉注射给药;大鼠眼眶静脉采血

据对尿酸酶Vm/Km的改变测定血浆尿酸酶抑制剂含量

动力学尿酸酶法测定血浆尿酸水平。结果:每天腹腔注射氧嗪酸钾持续7 d后

大鼠血浆尿酸可维持在0.40 mmol/L以上

但大鼠体内尿酸酶抑制剂也维持在很高水平。持续给予氧嗪酸钾21 d

每只大鼠给予0.5 U聚乙二醇修饰尿酸酶

配对t检验发现血浆尿酸有统计显著性下降

但降幅小于12%;给予低剂量或未经修饰尿酸酶则血浆尿酸水平无显著变化。结论:用尿酸酶抑制剂建立的高尿酸血症动物模型评价尿酸酶的降尿酸作用可靠性有限。

Abstract

Objective:To test the action of uricase to lower plasma urate in hyperuricemic rat model developed with oxonate. Methods: Potassium oxonate was given via intraperitoneal injection. Uricase after the filtration of bacteria was given via caudal vein injection. Blood samples were taken from orbital vein to determine equivalents of competitive inhibitors on uricase by their alteration on uricase Vm/Km and plasma uric acid levels by a direct kinetic uricase method. Results:After the continuous administration of potassium oxonate to rats once a day for one week

plasma uric acid levels were kept over 0.40 mmol/L

meanwhile uricase-inhibiting substances were kept at steady high levels. To hyperuricemic rats after continued administration of potassium oxonate for 3 weeks

the injection of 0.5 U uricase modified with monoether poly (ethylene glycol)reduced plasma uric acid levels by paired t-test analysis with an average degree less than 12%. The injection of unmodified uricase or lower levels of modified uricase produced no expected action. Conclusion:Because of serious interference from the uricase inhibitor in rat in vivo

hyperuricemic rats developed with uricase inhibitors show limited reliability for evaluating the pharmacological actions of uricases.

关键词

高尿酸血症尿酸酶氧嗪酸钾

Keywords

hyperuricemiauricasePotassiumoxonate

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