Effect of GRP78 on invasion and metastasis of human gastric carcinoma SGC7901 cells in vitro

YAN Xiao-li; et al

doi:10.13406/j.cnki.cyxb.2010.09.032

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Effect of GRP78 on invasion and metastasis of human gastric carcinoma SGC7901 cells in vitro

更新时间：2024-01-17

- Effect of GRP78 on invasion and metastasis of human gastric carcinoma SGC7901 cells in vitro
- Journal of Chongqing Medical University Vol. 35, Issue 9, Pages: 1332-1335(2010)
- 作者机构：
  
  重庆医科大学附属第一医院消化内科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.09.032
  CLC： R735.2
- Published：2010，
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[1]颜晓丽,高青,李禄椿.葡萄糖调节蛋白78对胃癌SGC7901细胞侵袭和转移的影响[J].重庆医科大学学报,2010,35(09):1332-1335.

YAN Xiao-li, et al. Effect of GRP78 on invasion and metastasis of human gastric carcinoma SGC7901 cells in vitro. [J]. Journal of Chongqing Medical University 35(9):1332-1335(2010)
[1]颜晓丽,高青,李禄椿.葡萄糖调节蛋白78对胃癌SGC7901细胞侵袭和转移的影响[J].重庆医科大学学报,2010,35(09):1332-1335. DOI： 10.13406/j.cnki.cyxb.2010.09.032.

YAN Xiao-li, et al. Effect of GRP78 on invasion and metastasis of human gastric carcinoma SGC7901 cells in vitro. [J]. Journal of Chongqing Medical University 35(9):1332-1335(2010) DOI： 10.13406/j.cnki.cyxb.2010.09.032.

摘要

目的:探讨钙离子螯合剂BAPTA-AM和钙离子载体A23187对胃癌SGC7901细胞葡萄糖调节蛋白78（Glucose regulated protein

GRP78）表达水平的影响

分析GRP78表达改变对胃癌细胞侵袭转移能力的影响。方法:BAPTA-AM和A23187处理细胞

RT-PCR和Western-blot检测3组细胞GRP78在mRNA和蛋白水平的表达

体外侵袭实验和划痕实验检测3组细胞的侵袭转移能力。结果:与对照组相比

BAPTA-AM组GRP78在mRNA和蛋白水平表达量降低

A23187组GRP78表达量升高;且BAPTA-AM组侵袭和转移能力明显低于对照组

而A23187组则高于对照组。结论:BAPTA-AM和A23187可分别下调和上调胃癌细胞GRP78的表达水平

GRP78的表达水平可能与胃癌细胞的侵袭和转移有关。

Abstract

Objective:To investigate the effect of BAPTA-AM and A23187 on GRP78 expression of gastric carcinoma SGC-7901 cells in vitro

and the effect of GRP78 on invasion and metastasis of these tumor cells.Methods:The SGC7901 cells were treated by BAPTA-AM and A23187.RT-PCR and Western blot were used to assess the expression of GRP78 at both mRNA and protein levels.Matrigel invasion assay and a scratch wound assay were used to assess the invasion and migration levels.Results:The expression of GRP78 at both mRNA and protein levels

the invasion and migration levels were decreased in the BAPTA-AM-treated cells and increased in A23187-treated cells as compared with those of control group.Conclusion:BAPTA-AM can down-regulate the expression of GRP78 in SGC7901 cells and A23187 can up-regulate it.The expression of GRP78 in SGC7901 cells may be associated with their migration and invasion levels.

关键词

胃癌葡萄糖调节蛋白78BAPTA-AM侵袭转移

Keywords

gastric carcinomaGRP78BAPTA-AMinvasionmetastasis

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