The damage of hyperglycemia to the neurons following traumatic brain injury

WANG Liang; et al

doi:10.13406/j.cnki.cyxb.2010.09.014

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The damage of hyperglycemia to the neurons following traumatic brain injury

更新时间：2024-01-17

- The damage of hyperglycemia to the neurons following traumatic brain injury
- Journal of Chongqing Medical University Vol. 35, Issue 9, Pages: 1327-1331(2010)
- 作者机构：
  
  重庆医科大学附属第一医院神经外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.09.014
  CLC： R651.1
- Published：2010，
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[1]王亮,陈亮,杨刚,唐文渊.创伤性脑损伤后高血糖对脑神经元损害作用的研究[J].重庆医科大学学报,2010,35(09):1327-1331.

WANG Liang, et al. The damage of hyperglycemia to the neurons following traumatic brain injury. [J]. Journal of Chongqing Medical University 35(9):1327-1331(2010)
[1]王亮,陈亮,杨刚,唐文渊.创伤性脑损伤后高血糖对脑神经元损害作用的研究[J].重庆医科大学学报,2010,35(09):1327-1331. DOI： 10.13406/j.cnki.cyxb.2010.09.014.

WANG Liang, et al. The damage of hyperglycemia to the neurons following traumatic brain injury. [J]. Journal of Chongqing Medical University 35(9):1327-1331(2010) DOI： 10.13406/j.cnki.cyxb.2010.09.014.

摘要

目的:探讨大鼠创伤性脑损伤后高血糖对神经元的损害作用。方法:成年雄性SD大鼠随机分成正常对照组

创伤性脑损伤（Traumatic brain injury

TBI）组和胰岛素治疗组。每组伤侧及健侧皮层设立自身对照。分别测定各组伤前伤后各时间点血糖值

逆转录-聚合酶链反应（RT-PCR）测定伤后伤侧及健侧皮层葡萄糖转运蛋白3（Glucose transporter3

GLUT-3）基因表达

Western-blot法测定伤后伤侧及健侧皮层GLUT-3蛋白表达

免疫荧光法测定伤后各组伤侧及健侧皮层单个神经元神经特异性烯醇化酶（Neuronspecificenolase

NSE）表达量和NSE阳性染色细胞数

longa评分、平衡木试验、网屏试验综合评定伤后各组神经功能。结果:正常对照组各时间点血糖值

GLUT-3表达量

单个神经元NSE表达量及NSE阳性细胞数均未见明显变化;TBI组动物伤后血糖升高

伤侧皮层GLUT-3表达增加

单个神经元NSE表达量增加而NSE阳性染色细胞数明显减少神经瘫裂较重;胰岛素治疗组伤后血糖变化不明显

伤后12、24、48、72hGLUT-3表达量

单个神经元NSE表达量及NSE阳性染色细胞数均明显多于TBI组神经瘫裂程度明显轻于TBI组。各组健侧皮层GLUT-3和单个神经元NSE表达量

NSE阳性染色细胞数均未见明显变化。结论:TBI后高血糖可加重神经元损伤

其机制可能与TBI后高血糖抑制伤后神经元GLUT-3的表达

增加伤后神经元葡萄糖代谢障碍有关。

Abstract

Objective:To explore the damage of hyperglycemia to the neurons.Methods:Adult male SD rats were randomly divided into 3 groups:normal control group

traumatic brain injury group and insulin treated group

the blood glucose concentrations of the rats were measured before injury and when they were killed.The method of RT-PCR was applied to detect the expression of GLUT-3 genes.The change of GLUT-3 protein was detected by western-blot.The changes of NSE and NSE positive-Staining cells were examined by immunofluorescence staining Longa

balance test and screen test score were used to assess neural fnuctional defect.Results:The blood glucose concentration only increased markedly in traumatic brain injury group.through the expression of GLUT-3 and single cell NSE increased in the injured cortical both in traumatic brain injury group and insulin treated group

there was much more expression of GLUT-3 and single cell NSE but less NSE positive-Staining cells and neural functional defect in the insulin treated group at 12

72 h after injury.Meanwhile there were no changes were found in the uninjured side of each group.Conclusion:The hyperglycemia after traumatic brain injury increased the damage to the neurons by decreasing the expression of GLUT-3.

关键词

创伤性脑损伤高血糖葡萄糖转运蛋白3神经元特异性烯醇化酶

Keywords

traumatic brain injuryhyperglycemiaGlucose transporter 3Neuronspecificenolase

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