Objective:To investigate the changes of EC、vessel wall and CRP after Central Vein Catheters at different times and determine a proper retention time.Methods:Models of central vein catheterization were established in 42 rabbits.And the animals were randomized into seven phases of six rabbits each according to catheter indwelling times of 0 d

1 week

2

3

4

5 week and 6 week.Immunoturbidimetry was used to detect the CRP and FIB of Blood specimens.After the animals were sacrificed

the catheter

access vein were dissected.The catheters were cultivated

Histological evaluation was performed by using standard light microscopy on Hematoxylin-eosin stained specimens.Scanning electron microscopy was used to observe the damage of endothelial cells.Results:①All catheters cultivated:(-);②The endothelia damage were more severe at 4 week than 3 week(P<0.05);③Inflammation reaction were found at 4week

thrombus and calcification at 5 week and 6 week;④The expression of CRP and FIB started the day after intubations and reached the maximum at 4week than 3week(P<0.05).Conclusion:After CVC

The damage of EC and inflammation reaction are severe at 4 week.