Comparison among several methods for apoptosis examination by Flow Cytometry

WU Jian-yong; et al

doi:10.13406/j.cnki.cyxb.2010.09.008

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Comparison among several methods for apoptosis examination by Flow Cytometry

更新时间：2024-01-17

- Comparison among several methods for apoptosis examination by Flow Cytometry
- Journal of Chongqing Medical University Vol. 35, Issue 9, Pages: 1386-1389(2010)
- 作者机构：
  
  重庆医科大学生命科学研究院
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.09.008
  CLC： R329.2
- Published：2010，
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[1]吴建勇,赵德璋.流式细胞仪检测细胞凋亡的几种方法的比较[J].重庆医科大学学报,2010,35(09):1386-1389.

WU Jian-yong, et al. Comparison among several methods for apoptosis examination by Flow Cytometry. [J]. Journal of Chongqing Medical University 35(9):1386-1389(2010)
[1]吴建勇,赵德璋.流式细胞仪检测细胞凋亡的几种方法的比较[J].重庆医科大学学报,2010,35(09):1386-1389. DOI： 10.13406/j.cnki.cyxb.2010.09.008.

WU Jian-yong, et al. Comparison among several methods for apoptosis examination by Flow Cytometry. [J]. Journal of Chongqing Medical University 35(9):1386-1389(2010) DOI： 10.13406/j.cnki.cyxb.2010.09.008.

摘要

目的:探索运用流式细胞仪来检测细胞凋亡的方法。方法:采用流式细胞仪对DNA含量、磷脂酰丝氨酸、线粒体膜电位、钙离子浓度的检测

分析细胞凋亡情况。结果:DNA含量测定存在一定误差;磷脂酰丝氨酸测定能准确反映细胞早晚期凋亡情况;线粒体膜电位、钙离子浓度测定能准确反映凋亡细胞的生理指标

如果结合形态学分析将更为客观。结论:通过比较实验方法

为将来采用流式细胞仪检测细胞凋亡提供更准确的实验方法。

Abstract

Objective:To explore the methods of using Flow Cytometry to examine the apoptosis.Methods:Flow Cytometry was used to examine the DNA content

the phosphatidyl serine

the mitcchondrial membrane potential and the calcium ion

the apoptosis was analyzed.Results:The DNA content had some deflexion.The phosphatidyl serine examination can reflect the early and advanced stage apoptosis punctually.The mitcchondrial membrane potential and the calcium ion examination can reflect the physiological index of apoptosis cell.But if combined with morphology the analysis will be more objective.Conclusion:Through comparing empirical method

we find more precise methods of using the Flow Cytometry to examine the apoptosis in the future.

关键词

流式细胞仪细胞凋亡DNA含量磷脂酰丝氨酸膜电位钙离子

Keywords

Flow CytometryapoptosisDNA contentPhosphatidyl serineMitcchondrial membrane potentialcalciumion

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