Effects of exogenetic recombinant protein S100A6 on proliferation and migration of 5 transformed cell lines and β-catenin expression level

MA Wen; et al

doi:10.13406/j.cnki.cyxb.2010.09.007

您当前的位置：

首页 >

文章列表页 >

Effects of exogenetic recombinant protein S100A6 on proliferation and migration of 5 transformed cell lines and β-catenin expression level

更新时间：2024-01-17

- Effects of exogenetic recombinant protein S100A6 on proliferation and migration of 5 transformed cell lines and β-catenin expression level
- Journal of Chongqing Medical University Vol. 35, Issue 9, Pages: 1321-1326(2010)
- 作者机构：
  
  1. 重庆医科大学生物医学工程系
  2. 重庆医科大学实验动物中心
  3. 重庆医科大学医学检验系临床检验诊断学、教育部重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.09.007
  CLC： R329
- Published：2010，
扫描看全文
[1]马闻,李星星,王胜,游莉,郭元元,徐兰兰,周兰.外源性重组S100A6蛋白对5株转化细胞系的增殖、迁移能力及β-catenin水平的影响[J].重庆医科大学学报,2010,35(09):1321-1326.

MA Wen, et al. Effects of exogenetic recombinant protein S100A6 on proliferation and migration of 5 transformed cell lines and β-catenin expression level. [J]. Journal of Chongqing Medical University 35(9):1321-1326(2010)
[1]马闻,李星星,王胜,游莉,郭元元,徐兰兰,周兰.外源性重组S100A6蛋白对5株转化细胞系的增殖、迁移能力及β-catenin水平的影响[J].重庆医科大学学报,2010,35(09):1321-1326. DOI： 10.13406/j.cnki.cyxb.2010.09.007.

MA Wen, et al. Effects of exogenetic recombinant protein S100A6 on proliferation and migration of 5 transformed cell lines and β-catenin expression level. [J]. Journal of Chongqing Medical University 35(9):1321-1326(2010) DOI： 10.13406/j.cnki.cyxb.2010.09.007.

摘要

目的:探讨S100A6对5种转化细胞系株（HEK293、9HTE、CHO、MEF和C3H10T1/2）的增殖、迁移能力和β-catenin水平的影响。方法:通过转化质粒pMoluc-GST-hS100A6到大肠杆菌BL21

经IPTG诱导表达重组S100A6蛋白

为后续实验提供材料;MTT法检测细胞增殖

细胞划痕和Transwell法检测细胞迁移

免疫细胞化学法及Western-blotting检测β-catenin表达水平。结果:①通过IPTG诱导表达

从大肠杆菌中收获了高纯度重组S100A6（GST-S100A6）;②外源性重组S100A6对作为阳性对照的肿瘤细胞系——人骨肉瘤细胞143B的增殖及迁移均有抑制作用（P<0.05）

而对5株转化细胞系的增殖及迁移均无显著影响（P>0.05）;③免疫细胞化学法和Western-blotting一致揭示重组S100A6可上调5株转化细胞系的β-catenin水平（P<0.05）。结论:S100A6对5株转化细胞系的增殖、迁移无明显影响

但可上调此5株转化细胞系Wnt/β-catenin水平。

Abstract

Objective:This study aimed to investigate effects of exogenetic recombinant protein S100A6 on 5 transformed cells(HEK293

9HTE

CHO

MEF and C3H10T1/2) and their β-catenin expression level.Methods:Plasmid pMoluc-GST-hS100A6 was transformed into E coli.BL21

in which S100A6 can be induced to express by IPTG;Cell proliferation was measured by MTT

cell migration was measured by cell scratch assay and Transwell assay and levels of β-catenin were measured by ICC and Western-blotting.Results:① High purity recombinant S100A6(GST-S100A6) was obtained from E Coli via induction of IPTG induction.②Exogenetic S100A6 can not only inhibit 143B cell lines(positive control) proliferation but also the migration(P<0.05).However

effects of different concentration of S100A6 cast on proliferation(P>0.05) and migration(P>0.05) of these 5 transformed cell lines were not statistically significant.③ S100A6 can up-regulate β-catenin level in these 5 transformed cell lines significantly(P<0.05).Conclusion:Effects of S100A6 on these 5 transformed cell lines were not significant neither on proliferation nor on migration

but it can up-regulate their β-catenin levels.

关键词

S100A6转化细胞系细胞增殖细胞迁移β-catenin

Keywords

S100A6Transformed cell linescell proliferationcell migrationβ-catenin

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of PLD2 gene silencing on proliferation and migration of colon cancer cells

Effect of exogenous S100A6 on human osteosarcoma cell line U2OS proliferation,apoptosis and β-catenin expression

Effects of SH3BP4 gene on the migration and proliferation of hepatocarcinoma cells

Construction of GSTZ1 knockout model based on CRISPR-Cas9 system and its effect on the proliferation and migration of hepatoma cells

Promotion of the proliferation of hepatocellular carcinoma cells by acetyl-CoA carboxylase 2 through cell cycle regulation

Related Author

Du Kunli

Liu Maoxi

Zhang Shouru

Wu Xingye

Zeng Li

Fu Zhongxue

CHEN Ying-hua

et al

Related Institution

Department of Integrated Traditional and Western Medicine,The First Affiliated Hospital of Chongqing Medical University

Department of Gastrointestinal Surgery,The First Affiliated Hospital of Chongqing Medical University

Department of Biomedical Engineering,Chongqing Medical University

美国芝加哥大学医学中心分子肿瘤研究室

Key Laboratory of Molecular Biology for Infectious Diseases，Ministry of Education，Chongqing Medical University

⁰