Prediction of the secondary structure and the B cell epitope of the extracellular domain of FSHR

SHEN Zi-gang; et al

doi:10.13406/j.cnki.cyxb.2010.09.006

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Prediction of the secondary structure and the B cell epitope of the extracellular domain of FSHR

更新时间：2024-01-17

- Prediction of the secondary structure and the B cell epitope of the extracellular domain of FSHR
- Journal of Chongqing Medical University Vol. 35, Issue 9, Pages: 1317-1320(2010)
- 作者机构：
  
  1. 第三军医大学免疫学教研室
  2. 第三军医大学西南医院妇产科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.09.006
  CLC： R392
- Published：2010，
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[1]申子刚,阎萍,何畏,陈正琼,何海洋,张记,杨霞,吴玉章,梁志清,李晋涛.FSHR胞外区抗原的二级结构预测和B细胞表位预测[J].重庆医科大学学报,2010,35(09):1317-1320.

SHEN Zi-gang, et al. Prediction of the secondary structure and the B cell epitope of the extracellular domain of FSHR. [J]. Journal of Chongqing Medical University 35(9):1317-1320(2010)
[1]申子刚,阎萍,何畏,陈正琼,何海洋,张记,杨霞,吴玉章,梁志清,李晋涛.FSHR胞外区抗原的二级结构预测和B细胞表位预测[J].重庆医科大学学报,2010,35(09):1317-1320. DOI： 10.13406/j.cnki.cyxb.2010.09.006.

SHEN Zi-gang, et al. Prediction of the secondary structure and the B cell epitope of the extracellular domain of FSHR. [J]. Journal of Chongqing Medical University 35(9):1317-1320(2010) DOI： 10.13406/j.cnki.cyxb.2010.09.006.

摘要

目的:分析卵泡刺激素受体（Follicle stimulating hormone receptor

FSHR）胞外区蛋白的二级结构

在线预测FSHR胞外区潜在的B细胞表位。方法:利用DNAStarProtein软件对FSHR胞外区蛋白的二级结构和表面特性进行分析

联合在线B细胞表位预测

预测FSHR胞外区蛋白可能的抗原表位。合成预测的B细胞表位肽

对其抗原性进行鉴定。结果:FSHR胞外区蛋白可能的抗原表位区域为17～34、42～44、116～122、142～147、179～193、239～241、266～270、279～282、288～307。选择4个区域合成肽段表位区域能与免疫抗血清发生抗原特异性反应。结论:应用多参数成功预测了FSHR胞外区蛋白抗原的B细胞表位。

Abstract

Objective:To predict the secondary structure and the B cell epitope for the extracellular domain of the follicle stimulating hormone receptor(FSHR).Methods:The secondary structure and surface properties of the extracellular domain of FSHR

such as physical and chemical properties

hydrophilicity

accessibility

antigenicity and plasticity

were analyzed by a variety of methods.The peptide of the epitopes was synthesized and used for coating streptavidin plate.The immunogenicity of the peptides was determined.Results:Many distinct antigenic epitopes in the extracellular domain of FSHR were identified by computation and online prediction

the possible location of which is in the regions of 17~34、42~44、116~122、142~147、179~193、239~241、266~270、279~282、288~307.Synthesized peptides have high specificity to the polyclonal antiboby.Conclusion:The B cell epitope for the extracellular domain of FSHR is successfully predicted.

关键词

FSHR胞外区B细胞表位二级结构抗原性

Keywords

Follicle stimulating hormone receptorB cell epitopeThe secondary stuctuerAntigentic

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