Effects of salvianic acid A(danshensu) on proliferation,activation and TGFβ/BMP receptors in hepatic stellate cells

WU Peng-yu; et al

doi:10.13406/j.cnki.cyxb.2010.09.004

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Effects of salvianic acid A(danshensu) on proliferation,activation and TGFβ/BMP receptors in hepatic stellate cells

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- Effects of salvianic acid A(danshensu) on proliferation,activation and TGFβ/BMP receptors in hepatic stellate cells
- Journal of Chongqing Medical University Vol. 35, Issue 9, Pages: 1344-1347(2010)
- 作者机构：
  
  重庆医科大学附属第二医院消化内科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.09.004
  CLC： R285
- Published：2010，
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[1]武鹏宇,戴立里,唐静,呙琳琳.丹参素对肝星状细胞增殖、活化及TGFβ/BMP受体表达的影响[J].重庆医科大学学报,2010,35(09):1344-1347.

WU Peng-yu, et al. Effects of salvianic acid A(danshensu) on proliferation,activation and TGFβ/BMP receptors in hepatic stellate cells. [J]. Journal of Chongqing Medical University 35(9):1344-1347(2010)
[1]武鹏宇,戴立里,唐静,呙琳琳.丹参素对肝星状细胞增殖、活化及TGFβ/BMP受体表达的影响[J].重庆医科大学学报,2010,35(09):1344-1347. DOI： 10.13406/j.cnki.cyxb.2010.09.004.

WU Peng-yu, et al. Effects of salvianic acid A(danshensu) on proliferation,activation and TGFβ/BMP receptors in hepatic stellate cells. [J]. Journal of Chongqing Medical University 35(9):1344-1347(2010) DOI： 10.13406/j.cnki.cyxb.2010.09.004.

摘要

目的:观察丹参素对肝星状细胞增殖、活化及TGFβ

BMP受体表达的影响

进一步探索丹参素抗肝纤维化的机制。方法:用不同浓度（0.25、0.125、0.0625mmol/L）的丹参素作用于经TGFβ（15ng/ml）处理人肝星状细胞LX-248h

CCK8法检测其对细胞增殖的影响

免疫化学染色法观察α平滑肌动蛋白（α-SMA）的表达

RT-PCR检测TGFβⅠ、Ⅱ型受体

BMPⅡ型受体mRNA的表达。结果:TGFβ15ng/ml处理48h后

LX-2细胞的增殖与细胞对照组无明显差异

高

中

低浓度丹参素处理组的细胞增殖受到浓度依赖性抑制（P<0.01）。TGFβ1诱导的细胞活化也可被丹参素浓度依赖性抑制。丹参素可浓度依赖性减少TβRⅠ、Ⅱ和增加BMPRⅡmRNA表达（P<0.01）。结论:丹参素可通过抑制肝星状细胞增殖和TGFβ1诱导的肝星状细胞活化

负性调控肝纤维化过程

其机制可能为抑制TGFβ受体表达并拮抗TGFβ引起的BMP受体表达下调

进而影响下游Smad分子。

Abstract

Objective:To investigate the effects of salvianic acid A(Danshensu) on proliferation

activation

expressions of TGFβand BMP receptors in hepatic stellate cells

and the possible mechanism.Methods:LX-2 cells were cultured in vitro

and devided into 5 groups:normal control as group A

5 ng/ml TGFβ1 treatment as group B

and salvianic acid a groups with 0.25

0.125

0.0625 mmol/L of salvianic acid A and 5 ng/ml TGFβ1 as groups C1-3.After 48h treatment

cell proliferation was measured by Cell Counting Kit-8.The expressions of α-smooth muscle actin(α-SMA) was detected by the immunocytochemical assay.Expressions of TGFβreceptor Ⅰ

Ⅱ(TβRⅠ

Ⅱ) and BMP receptorⅡ(BMPRⅡ) mRNAs

were detected by RT-PCR.Results:The proliferation of LX-2 treated by TGF-β1 was not significant different from the control group

salvianic acid A suppressed the proliferation of TGF-β1 treating LX-2 in a dose-dependent manner(P<0.01)

and also the TGF-β1 inducing activation.Expressions of TGFβreceptors decreased and expressions of BMP receptors increased dose-dependently(P<0.01) after treated by salvianic acid A for 48 h.Conclusion:salvianic acid A inhibits the proliferation and activation of HSCs possibly by down-regulating the TβRⅠ

Ⅱ expressions and antagonizing TGFβinduced down-regulating the BMPRⅡ expressions.

关键词

肝纤维化肝星状细胞骨形态发生蛋白丹参素

Keywords

Hepatic fobrosishepatic stellate cellsBMPDanshensu

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