Objective:To observe the intervention effect on expression of apportosis and related factors of apportosis in spleen-qi deficiency rats treated by electroacupuncture in tissue oxidized or anti-oxidized injury and to investigate the protective effect and mechanism on spleen-qi deficiency treated by electroacupuncture.Methods:The rats’ syndrome models of deficiency of spleen-qi were made by the method of compound causes of disease according to the TCM ’s theory of injury due to diet and overstrain.Appearance of rats were observed:tissue ultrastructural changes measured with methods of Hemtoxycin-eosin(HE).The activites of liver superoxide dismutase(SOD) and the contents of liver malondial dehyde(MDA) were determined by colorimetry.The apoptosis cells in liver tissue were tested by TdT—mediated digoxin—dUTP nick end labeling(TUNEL).Results:We discuss the relationships between normal and model group

on the activity of SOD and the contents of MDA in liver of spleen-qi deficiency rats.The resu1t was that MDA of model group was more than normal group(P<0.01) and the activity of SOD stronger of normal group(P<0.01).The apoptosis index was increased obviously in rat with liver injury induced by spleen-qi deficiency(P<0.01).The resu1t was decreased that the contents of MDA and the apoptosis index in live treated by electroacupuncture.The activity of SOD was stronger of electroacupuncture group.Conclusion:The Mechanism of electroacupuncture to spleen-qi deficiency maybe is regarded to the effect of Anti-Oxidanted injury and Anti-apoptesis in tissue cells.