The relation of the content changes of cardiac muscle Myoglobin(Mb) mRNA to degradation time in rats after death

WANG Quan-fang; et al

doi:10.13406/j.cnki.cyxb.2010.08.046

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The relation of the content changes of cardiac muscle Myoglobin(Mb) mRNA to degradation time in rats after death

更新时间：2024-01-17

- The relation of the content changes of cardiac muscle Myoglobin(Mb) mRNA to degradation time in rats after death
- Journal of Chongqing Medical University Vol. 35, Issue 8, Pages: 1205-1208(2010)
- 作者机构：
  
  重庆医科大学基础医学院法医学教研室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.08.046
  CLC： D919
- Published：2010，
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[1]王全芳,邓世雄,蒋朴,黄海,李虹.大鼠死后心肌肌红蛋白mRNA的含量变化与死亡时间的相关性研究[J].重庆医科大学学报,2010,35(08):1205-1208.

WANG Quan-fang, et al. The relation of the content changes of cardiac muscle Myoglobin(Mb) mRNA to degradation time in rats after death. [J]. Journal of Chongqing Medical University 35(8):1205-1208(2010)
[1]王全芳,邓世雄,蒋朴,黄海,李虹.大鼠死后心肌肌红蛋白mRNA的含量变化与死亡时间的相关性研究[J].重庆医科大学学报,2010,35(08):1205-1208. DOI： 10.13406/j.cnki.cyxb.2010.08.046.

WANG Quan-fang, et al. The relation of the content changes of cardiac muscle Myoglobin(Mb) mRNA to degradation time in rats after death. [J]. Journal of Chongqing Medical University 35(8):1205-1208(2010) DOI： 10.13406/j.cnki.cyxb.2010.08.046.

摘要

目的:研究大鼠心肌肌红蛋白（Myoglobin

Mb）mRNA的降解规律

探讨其与死亡时间（Postmortem interval

PMI）的关系。方法:在25℃±1℃

65%～70%温湿度控制系统下

通过采用RT-PCR技术检测大鼠死后心肌MbmRNA在体保存和离体保存条件下各相应时间段的含量变化

用SPSS17.0软件对数据进行分析。结果:大鼠死后心肌MbmRNA随死亡（离体）时间延长而逐渐降低

且与死亡（离体）时间呈线性关系

但在体保存要比离体保存降解慢。结论:MbmRNA的降解呈时间相关性

在体与离体条件下的降解规律并不完全一致;MbmRNA有望用于推断较早期死后经过时间。

Abstract

Objective:To study the degradation regularity of Moglobin (Mb) mRNA in rats and to explore the relationship between the time of death.Methods:Using RT-PCR to detect the Mb mRNA levels of the corresponding time changes in rat heart after death at 25℃±1℃

65%～70%temperature and humidity control system

and use SPSS 17.0 software for data analysis.Results:The contents of the Mb mRNA in rats gradually decreased with the lengthening of the postmortem interval

and it had a proximate linear relationship with the postmortem interva1.The degradation of Mb mRNA is lower than that in vitro.Conclusion:Mb mRNA was time-related degradation

and the degradation under the conditions of the law was not entirely consistent in vivo and vitro;Mb could be used for estimating the earlier postmortem interva1.

关键词

法医病理学死亡时间心肌肌红蛋白逆转录-聚合酶链式反应

Keywords

forensic pathologypostmortem intervalMyoglobin(Mb)RT-PCR

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