Effects and mechanisms of insulin postconditioning on myocardial protection in isolated rat hearts

WANG Wei-xin; et al

doi:10.13406/j.cnki.cyxb.2010.08.040

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Effects and mechanisms of insulin postconditioning on myocardial protection in isolated rat hearts

更新时间：2024-01-17

- Effects and mechanisms of insulin postconditioning on myocardial protection in isolated rat hearts
- Journal of Chongqing Medical University Vol. 35, Issue 8, Pages: 1163-1166(2010)
- 作者机构：
  
  重庆医科大学附属第一医院胸心外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.08.040
  CLC： R96
- Published：2010，
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[1]王为新,杨双强,辛东,欧袁.胰岛素后处理对离体大鼠心肌的保护作用及其机制[J].重庆医科大学学报,2010,35(08):1163-1166.

WANG Wei-xin, et al. Effects and mechanisms of insulin postconditioning on myocardial protection in isolated rat hearts. [J]. Journal of Chongqing Medical University 35(8):1163-1166(2010)
[1]王为新,杨双强,辛东,欧袁.胰岛素后处理对离体大鼠心肌的保护作用及其机制[J].重庆医科大学学报,2010,35(08):1163-1166. DOI： 10.13406/j.cnki.cyxb.2010.08.040.

WANG Wei-xin, et al. Effects and mechanisms of insulin postconditioning on myocardial protection in isolated rat hearts. [J]. Journal of Chongqing Medical University 35(8):1163-1166(2010) DOI： 10.13406/j.cnki.cyxb.2010.08.040.

摘要

目的:探讨胰岛素（Insulin）后处理对离体大鼠心肌的保护作用及其可能机制。方法:将32只Wistar大鼠随机分成4组

对照组（Control组）

缺血再灌注组（I/R组）

低剂量胰岛素后处理组（L-POST组）

高剂量胰岛素后处理组（H-POST组）

每组8只。Control组开胸取左心室肌作为缺血前对照;I/R组、L-POST组、H-POST组建立离体心脏Langendorff-Neely灌注模型

预灌注K-H缓冲液30min后

灌注4℃STH-2心脏停搏液

停搏心脏并低温维持30min

复灌K-H缓冲液60min后

取左心室肌备用;L-POST组和H-POST组于复灌期给予含胰岛素的K-H液灌注。采用免疫组化和Western blot分别检测大鼠心肌内皮型一氧化氮合酶（Endothe lial nitric oxide synthase

eNOS）、蛋白激酶C（Proteinkinase C

PKC）的分布和量的变化;TUNEL法检测心肌细胞凋亡指数（Apoptotic index

AI）。结果:与Control组相比

I/R组、L-POST组、H-POST组内皮型一氧化氮合酶（Endothelial nitric oxide synthase

eNOS）、蛋白激酶C（Protein kinase C

PKC）表达量明显增多（P<0.05）

AI明显增高（P<0.05）;与I/R组相比

L-POST组和H-POST组eNOS、PKC表达量明显增加（P<0.05）

AI明显减少（P<0.05）;与L-POST组相比

H-POST组eNOS、PKC表达量增加（P<0.05）

AI明显减少（P<0.05）。结论:胰岛素后处理具有减轻心肌缺血/再灌注损伤的作用

其机制可能与增加eNOS、PKC表达

抑制细胞凋亡有关

高剂量胰岛素对缺血/再灌注心肌细胞较低剂量胰岛素具有更显著的保护作用。

Abstract

Objective:To explore the effects and mechanisms of insulin postconditioning on myocardial protection in isolated rat hearts.Methods:32 Wistar rats were randomly divided intocontrol group

ischemia/reperfusion group(I/R group)

low dose insulin postconditioning group(L-POST group)and high dose insulin postconditioning group(H-POST group)

with 8 rats in each group.Langendorff and neely perfusion models were steadily established

then the rat hearts were arrested by cardioplegia(St.Thomas No.2 solution

STH-2)after perfusion for 30 min.These arrested hearts were remained for 30 min at 4℃and then underwent reperfusion for 60 min.In L-POST group and H-POST group

the arrested hearts were reperfused by Krebs-Henseleit buffer solution(K-H)with insulin.The content changes of myocardial eNOS and PKC were detected by immunohistochemistry and Western blo(tWB)

the AI(Apoptosis index

AI)of cardiomyocyte was detected by TUNEL.Results:Compared with control group

higher expression of eNOS and PKC and higher AI were detected in I/R group、L-POST group and H-POST group (P<0.05);compared with I/R group

higher expression of eNOS and PKC and lower AI were detected in L-POST group and H-POST group(P<0.05);compared with L-POST group

higher expression of eNOS and PKC

and lower xpression of AI were detected in H-POST group(P<0.05).Conclusion:The insulin postconditioning could protect heart from myocardial ischemia/reperfusion injury

which might be associated with increased eNOS

PKC

and inhibited apoptosis.The effect of high dose insulin postconditioning is better than that of the low dose.

关键词

胰岛素后处理缺血/再灌注损伤内皮型一氧化氮合酶蛋白激酶C凋亡

Keywords

Insulin postcondi tioningischemia/reperfusion injuryeNOSPKCapoptosis

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