Objective:To study the the mechanismof effect of glucocorticoids and BCGvaccine in the treatment of secretory otitis media. Methods:24 BALB/C mice were divided into 4 groups of 6

The OVA、BCG and GCS groups were immunized with OVA/alum and challenged with OVA.BCG group was injected with BCG 0.02 mg intraperitoneal injection 3 days before being challenged

GCS group was given the oral inhalation prednisone 1 mg for 3 days before being stimulated.And the other two groups have non-intervention

2 days later the mice were killed all

We analysed the pathology observation of the middle ear mucosa

the expression of cytokines such as IL-4、IL-5、 IFN-γand IL-12 by immunohistochemistry.Results:BCG Group

GCS group of middle ear mucosa inflammatory response than the OVA group was significantly reduced

and the GCS group

compared with BCG Group

also significantly reduce the inflammatory.Th1-type cytokines IL-12

IFN-γwas significantly increased than the OVA group

(P<0.05);while Th2-type cytokines IL-4

IL-5 was significantly reduced

(P<0.05).Conclusion:BCG is an effective immunomodulator

probably by regulating the Th1/Th2 balance.And glucocorticoids are still the the most effective drugs in the treatment of allergic inflammatory disease.The study provides the basis for the clinical treatment of secretory otitis media.