Establishment of multidrug resistant animal model in mice CT26 colon cancer cells

GUI Lin-ling; et al

doi:10.13406/j.cnki.cyxb.2010.08.036

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Establishment of multidrug resistant animal model in mice CT26 colon cancer cells

更新时间：2024-01-17

- Establishment of multidrug resistant animal model in mice CT26 colon cancer cells
- Journal of Chongqing Medical University Vol. 35, Issue 8, Pages: 1224-1227(2010)
- 作者机构：
  
  重庆医科大学附属儿童医院普外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.08.036
  CLC： R735.3
- Published：2010，
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[1]桂琳玲,金先庆,罗庆.小鼠CT26结肠癌耐药动物模型的建立[J].重庆医科大学学报,2010,35(08):1224-1227.

GUI Lin-ling, et al. Establishment of multidrug resistant animal model in mice CT26 colon cancer cells. [J]. Journal of Chongqing Medical University 35(8):1224-1227(2010)
[1]桂琳玲,金先庆,罗庆.小鼠CT26结肠癌耐药动物模型的建立[J].重庆医科大学学报,2010,35(08):1224-1227. DOI： 10.13406/j.cnki.cyxb.2010.08.036.

GUI Lin-ling, et al. Establishment of multidrug resistant animal model in mice CT26 colon cancer cells. [J]. Journal of Chongqing Medical University 35(8):1224-1227(2010) DOI： 10.13406/j.cnki.cyxb.2010.08.036.

摘要

目的:化疗药物诱导建立小鼠CT26结肠癌多药耐药（Multidrug resistance

MDR）动物模型。方法:建立小鼠CT26结肠癌皮下肿瘤模型

5-氟尿嘧啶和阿霉素联合化疗

MTT法绘制化疗前后细胞株的生长曲线、计算倍增时间及对各化疗药物敏感性及耐药指数（Resistance index

RI）

RT-PCR检测化疗前后MDR1b mRNA表达情况

流式细胞技术检测多药耐药蛋白（P-glycoprotein

P-gp）外排功能。结果:耐药株的倍增时间比亲本细胞株长

化疗后肿瘤对放线菌素D（ACT）、阿霉素（ADM）、长春新碱（VCR）、羟基喜树碱（HCPT）、5-氟尿嘧啶（5-Fu）、柔红霉素（DAM）、依托泊甙（VP16）的RI分别为29.3、26.4、10.9、7.0、5.8、3.0、2.8

敏感性降低。耐药株MDR1b mRNA表达水平比亲本细胞株高

对柔红霉素的外排能力明显高于亲本细胞株。结论:本实验成功建立肿瘤多药耐药的动物模型

为化疗后肿瘤继发多药耐药问题的研究提供动物模型

为提高化疗效果研究奠定基础。

Abstract

Objective:To establish the multidrug resistant animal model of CT26 colon cancercells in mice with the use of chemotherapy. Methods:CT26 colon cancer cells were injected subcutaneously into the right oxters of the mice.Fluorouracil and doxorubicin were combined into chemotherapy.Drown growth curve and calculate doubling generation time

chemotherapeutics drug sensitivity and drug fast index number of the tumor cells before and after chemotherapy were evaluated by MTT method.Levels of MDR1b mRNA were measured by RT-PCR.Eliminate function of P-gp were measured by flow cytometer.Results:Doubling generation time of the persister was higher than the parent cells.Drug resistance indexes were 29.3

26.4

10.9

7.0

5.8

3.0、and 2.8 respectively to ACT

ADM

VCR

HCPT

5-Fu

DAM

and VP16 after chemotherapy and the drug sensitivity were lower.The levels of MDR1b mRNA increased after chemotherapy and P-gp excretive ability was higher than that of no chemotherapy group.Conclusion:The multidrug resistant animal model has established successfully

which provided animal model for our study and research in the acquired multidrug resistance

and established groundwork for the research in increasing the chemotherapy effect.

关键词

多药耐药动物模型化疗

Keywords

Drugresistanceanimal modelchemotherapy

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