Selection and identification of differential proteins in plasma/serum with hepatocellular carcinoma by proteomic techniques

SHANG Jing-chuan; et al

doi:10.13406/j.cnki.cyxb.2010.08.034

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Selection and identification of differential proteins in plasma/serum with hepatocellular carcinoma by proteomic techniques

更新时间：2024-01-17

- Selection and identification of differential proteins in plasma/serum with hepatocellular carcinoma by proteomic techniques
- Journal of Chongqing Medical University Vol. 35, Issue 8, Pages: 1212-1216(2010)
- 作者机构：
  
  1. 重庆医科大学药学院药物分析教研室
  2. 重庆医科大学分子医学与肿瘤研究中心
  3. 重庆医科大学生命科学研究院
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.08.034
  CLC： R735.7
- Published：2010，
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[1]尚京川,易发平,吴民军,母昭德.蛋白质组学技术筛选肝癌血浆/血清中的差异蛋白质[J].重庆医科大学学报,2010,35(08):1212-1216.

SHANG Jing-chuan, et al. Selection and identification of differential proteins in plasma/serum with hepatocellular carcinoma by proteomic techniques. [J]. Journal of Chongqing Medical University 35(8):1212-1216(2010)
[1]尚京川,易发平,吴民军,母昭德.蛋白质组学技术筛选肝癌血浆/血清中的差异蛋白质[J].重庆医科大学学报,2010,35(08):1212-1216. DOI： 10.13406/j.cnki.cyxb.2010.08.034.

SHANG Jing-chuan, et al. Selection and identification of differential proteins in plasma/serum with hepatocellular carcinoma by proteomic techniques. [J]. Journal of Chongqing Medical University 35(8):1212-1216(2010) DOI： 10.13406/j.cnki.cyxb.2010.08.034.

摘要

目的:建立分析肝癌患者血浆/血清中低丰度差异表达蛋白质的双向凝胶电泳（2-DE）方法。方法:以10例肝癌患者血浆、8例肝癌患者血清及8例正常志愿者血浆和血清为研究对象

选用安捷伦免疫亲和色谱Agilent MARS Spin Cartridge去除血浆中6种主要高丰度蛋白

2-DE分离血浆/血清低丰度蛋白质

差异蛋白质斑点经酶解后

MALDI-TOF/MS检测的肽指纹谱（Peptide mass fingerprinting

PMF）在NCBInr数据库中进行搜索。结果:10例肝癌患者血浆样品中共鉴定出有20种差异蛋白

其中18种表达上调

2种表达。8例肝癌患者血清样品中共鉴定出23种差异蛋白

其中17种蛋白表达上调

6种蛋白表达下调。在肝癌患者血清和血浆组中共同识别到2种差异蛋白质。结论:血浆组中识别到的指环蛋白34（RING fingerprotein 34）

威尔姆肿瘤1-关联蛋白（Wilms’ tumor1-associating）

CD147抗原

卵泡前体FS（Follistatin precursor

FS）蛋白和血清组中识别到的蛋白激酶NYD-SP9（Protein kinase NYD-SP9）

肿瘤坏死因子受体超家族成员19前体（Tumor necrosis factor receptor superfamily member 19 precurso）r

组蛋白键合蛋白RBBP4（Histone-binding protein RBBP4）

FADD蛋白

26S蛋白酶调控亚基P28（26S proteasome regulatory subunitp28）

CD82抗原

自体吞噬蛋白5（Autophagyprotein5）等蛋白有望成为（Hepato celluar careinoma

HCC）的特异蛋白。

Abstract

Objective:To establish a two-dimensional polyacrylamide gel electrophoresi(s2-DE)technique forplasma/serumproteomics. Methods:Plasmas from 8 healthy volunteers and 10 with hepatocellular carcinoma(HCC)

and sera from 8 HCC were collected.After six proteins of the highest abundance in plasma/sera were depleted by the Multiple Affinity Removal System(MARS)Technologies from Agilent

plasma/sera were subjected to 2-DE.After comparing

analyzing and identifying by the software PD Quest(7.4

the enzyme-digested products of differential expression proteins were analyzed by MALDI-TOF-MS.The Peptide mass fingerprinting(PMF) data were obtained and used to identify proteins through NCBInr data bank.Results:In comparison with the healthy volunteers

20 differential proteins were identified in the plasmas of the HCC patients

among which 18 protein expressions were up-regulated and 2 down-regulated.In the sera of the cancer patients

23 differential proteins were identified

among which 17 protein expressions were up-regulated and 6 down-regulated.Tumor necrosis factor-inducible protein TSG-14 and FADD protein were both identified in the plasmas and sera of the cancer patients.Conclusion:The differential proteins

including RING finger protein 34

Wilms’tumor 1-associating protein

CD147 antigen

Tumor necrosis factor-inducible protein TSG-14 from plasmas group and Protein kinase NYD-SP9

Tumor necrosis factor receptor super family member 19 precursor

Histone-binding protein RBBP4

FADD protein

26S protease regulatory subunit p28

CD82 antigen

Autophagy protein 5 from sera group may be used as markers for HCC in blood.

关键词

肝细胞癌血浆/血清蛋白质组学双向电泳MALDI-TOF/MS

Keywords

Hepatocellular carcinoma(HCC)Plasma/seraproteomicsTwo-dimensional polyacrylamide gel electrophoresisMALDI-TOF-MS

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