Effect of simulated CO<sub>2</sub> pneumoperitoneum on cell proliferation of human renal carcinoma 786-O cells line

DENG Xin-jun; et al

doi:10.13406/j.cnki.cyxb.2010.08.033

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Effect of simulated CO₂ pneumoperitoneum on cell proliferation of human renal carcinoma 786-O cells line

更新时间：2024-01-17

- Effect of simulated CO₂ pneumoperitoneum on cell proliferation of human renal carcinoma 786-O cells line
- Journal of Chongqing Medical University Vol. 35, Issue 8, Pages: 1201-1204(2010)
- 作者机构：
  
  重庆医科大学附属第二医院泌尿外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.08.033
  CLC： R737.11
- Published：2010，
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[1]邓新军,胡自力,姚善华.模拟二氧化碳气腹对人肾癌786-O细胞体外增殖的影响[J].重庆医科大学学报,2010,35(08):1201-1204.

DENG Xin-jun, et al. Effect of simulated CO₂ pneumoperitoneum on cell proliferation of human renal carcinoma 786-O cells line. [J]. Journal of Chongqing Medical University 35(8):1201-1204(2010)
[1]邓新军,胡自力,姚善华.模拟二氧化碳气腹对人肾癌786-O细胞体外增殖的影响[J].重庆医科大学学报,2010,35(08):1201-1204. DOI： 10.13406/j.cnki.cyxb.2010.08.033.

DENG Xin-jun, et al. Effect of simulated CO₂ pneumoperitoneum on cell proliferation of human renal carcinoma 786-O cells line. [J]. Journal of Chongqing Medical University 35(8):1201-1204(2010) DOI： 10.13406/j.cnki.cyxb.2010.08.033.

摘要

目的:通过建立体外模拟CO2气腹环境

研究CO2气腹对人肾癌786-O细胞增殖的影响。方法:建立体外模拟CO2气腹环境

以14mmHg气压对人肾癌786-O细胞作用不同时间（2、4h）

以培养于常规条件下肾癌细胞为对照

用MTT法检测细胞增殖情况;流式细胞仪检测细胞周期分布和细胞凋亡率;免疫细胞化学法检测增殖细胞核抗原（PCNA）和Survivin蛋白表达水平。结果:与对照组比较

CO2气腹处理后的两组人肾癌786-O细胞增殖速度明显加快（P<0.05）

且48h后细胞增殖速度的加快与气腹作用时间呈直线相关（R2=1

P<0.01）;细胞周期中处于增殖期的细胞比例显著增高（P<0.01）;PCNA表达明显增强（P<0.01）;细胞凋亡率亦显著减少（P<0.05）

且凋亡率的减少与气腹作用时间呈直线相关（R2=1

P<0.01）;Survivin表达显著增强（P<0.01）。结论:体外模拟CO2气腹环境

可促进人肾癌786-O细胞增殖

且随着接触CO2时间的延长

肾癌786-O细胞增殖速度加快。

Abstract

Objective:Tostudythe effects ofsimulated carbon dioxide pneumoperiton eumenvironment on cell proliferation of human renal carcinoma 786-O cells in vitro.Methods:The renal carcinoma 786-O cells were exposed to simulated CO2 pneumoperitoneum environment for different time periods(2 h or 4 h)under the pressure of 14 mmHg and those cultured in normal experimental condition as control

then proliferation viability of 786-O cells was observed by MTT assay close behind the exposure;cell cycle and apoptotic rate were detected by flow cytometry

the protein expression of PCNA and Survivin were examined by immunocytochemistry 48 h after exposure respectively.Results:Compared with the control group

Both of the treated groups showed cell proliferation was significantly increased (P<0.05)

and the increasing had a linear relationship with exposure time(R2=1

P<0.01);The proliferative index(PI)showed significant difference from that of control group(P<0.01);In cell cycle

the percentages of S-phase and G2/M phase were significantly higher in treated groups than those in control group(P<0.05);PCNA protein was found to be significantly upregulated in treated groups (P<0.05).The apoptotic rates were significantlydecreased(P<0.01)

andthe decreasinghadalinearrelationshipwithexposure time;the expressions of Survivin

which related with apoptosis

were significantly increased(P<0.01).Conclusion:Carbon dioxide could promote the proliferation of human renal carcinoma 786-O cells

and the proliferation would be sped up with the prolongation of exposure time.

关键词

肾肿瘤二氧化碳气腹增殖凋亡

Keywords

Tumor of kidneyCarbon dioxidepneumoperitoneumproliferationapoptosis

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Related Institution

Department of Gastrointestinal Surgery，The Third Affiliated Hospital of Zunyi Medical University/ The First People’s Hospital of Zunyi

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Department of Gynaecology and Obstetrics,The First Affiliated Hospital of Chongqing Medical University

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Effect of simulated CO2 pneumoperitoneum on cell proliferation of human renal carcinoma 786-O cells line

DOI：10.13406/j.cnki.cyxb.2010.08.033

摘要

Abstract

关键词

Keywords

references

Effect of simulated CO₂ pneumoperitoneum on cell proliferation of human renal carcinoma 786-O cells line