Apigenin-induced apoptosis of bladder cancer cell BIU-87 and its mechanism

YAO Shan-hua; et al

doi:10.13406/j.cnki.cyxb.2010.08.032

您当前的位置：

首页 >

文章列表页 >

Apigenin-induced apoptosis of bladder cancer cell BIU-87 and its mechanism

更新时间：2024-01-17

- Apigenin-induced apoptosis of bladder cancer cell BIU-87 and its mechanism
- Journal of Chongqing Medical University Vol. 35, Issue 8, Pages: 1194-1197(2010)
- 作者机构：
  
  重庆医科大学附属第二医院泌尿外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.08.032
  CLC： R737.14
- Published：2010，
扫描看全文
[1]姚善华,胡自力,邓新军.芹菜素诱导膀胱癌细胞株BIU-87凋亡及机制的研究[J].重庆医科大学学报,2010,35(08):1194-1197.

YAO Shan-hua, et al. Apigenin-induced apoptosis of bladder cancer cell BIU-87 and its mechanism. [J]. Journal of Chongqing Medical University 35(8):1194-1197(2010)
[1]姚善华,胡自力,邓新军.芹菜素诱导膀胱癌细胞株BIU-87凋亡及机制的研究[J].重庆医科大学学报,2010,35(08):1194-1197. DOI： 10.13406/j.cnki.cyxb.2010.08.032.

YAO Shan-hua, et al. Apigenin-induced apoptosis of bladder cancer cell BIU-87 and its mechanism. [J]. Journal of Chongqing Medical University 35(8):1194-1197(2010) DOI： 10.13406/j.cnki.cyxb.2010.08.032.

摘要

目的:探讨芹菜素（Apigenin）在体外诱导膀胱癌细胞株BIU-87凋亡的作用及其机制。方法:MTT法测定不同浓度芹菜素作用后BIU-87细胞的生长情况;流式细胞术检测细胞周期情况;Annexin V-FITC/PI双染流式细胞术观察芹菜素作用后细胞的凋亡情况;RT-PCR法检测Survivin mRNA表达的变化;免疫细胞化学SP法检测Survivin和Caspase-3蛋白表达的变化。结果:①MTT法证实芹菜素能明显抑制膀胱癌细胞株BIU-87的生长。②22.03μmol/L芹菜素作用72h后

流式细胞仪检测发现芹菜素可使BIU-87细胞阻滞在G2/M期;流式细胞术双染法检测细胞凋亡显示:芹菜素可使BIU-87细胞发生凋亡。③RT-PCR检测发现芹菜素可使BIU-87细胞Survivin mRNA表达显著减少（P<0.05）

免疫细胞化学SP法检测显示Survivin蛋白表达亦显著减少（P<0.05）

Caspase-3蛋白表达则明显增加（P<0.01）。结论:芹菜素在体外可抑制BIU-87细胞增殖

诱导其发生凋亡。芹菜素诱导BIU-87细胞发生凋亡的生物学效应可能与Survivin的表达下降

Saspase-3的表达上升有关。

Abstract

Objective:To investigate the apoptosis of bladder cancer cell BIU-87 induced by apigenin in vitro and its mechanism. Methods:The cell proliferation was detected by MTT assay;the cell cycle was measured by flow cytometry;the cell apoptosis was observed by flow cytometry with Annexin V-FITC/PI double staining;the expression of survivin mRNA was detected by RT-PCR

and the expressions of survivin protein and caspase-3 protein were detected by immunocytochemistry SP assay.Results:MTT showed that apigenin effectively inhibited the proliferation of BIU-87 cells.②After 72 hours of 22.03μmol/L apigegin treatment

it could arrest the BIU-87 cell in G2/M phase which was measured by flow cytometry

and it could make the cell apoptosis which was showed by flow cytometry with Annexin V-FITC and PI.③Compared with the control group

the expression of survivin mRNA was significantly decreased (P<0.05)

andsowas the expressionofsurvivinprotein(P<0.05)

while thatofcaspase-3 protein was marklyupregulated(P<0.01)in the apigenin group.Conclusion:Apigenin inhibits the proliferation and induces the apoptosis of BIU-87 cells.The molecular mechanism of apigenin inducing the apoptosis of BIU-87 cells may be associated with its action of down-regulating the expression of survivin

and up-regulating the expression of caspase-3.

关键词

芹菜素膀胱癌细胞凋亡

Keywords

Apigeninbladder cancerapoptosis

references

Views

166

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Glycyrrhiza uralensis Fisch. endophytes combined with cisplatin on proliferation and apoptosis of A549 cells

Milk fat globule-EGF factor Ⅷ attenuates apoptosis and permeability damage of the intestinal mucosa in rats with obstructive jaundice

Value of inflammatory，pyroptosis，and ferroptosis biomarkers in the treatment and prognosis of bladder cancer

Correlation between serum KLF5 content and positive lymph node ratio and its predictive value for prognosis of patients with bladder cancer after radical operation

Value of inflammatory biomarkers in predicting the outcome of patients with muscle-invasive bladder cancer receiving different neoadjuvant treatment regimens

Related Author

Zhang Shanglong

Zhang Nan

Lian Xiaolong

Ye Liqiao

Ma Quhuan

Deng Yi

Guo Yong

Tang Yongliang

Related Institution

College of Pharmacy，Gansu University of Chinese Medicine

Department of Hepatobiliary Pancreatic Surgery,Daping Hospital,Army Medical University

Department of Hepatobiliary Pancreatic Surgery,People’s Hospital of Chongqing Liang Jiang New Area

Department of Urology,Hunan Cancer Hospital/The Affiliated Cancer Hospital of Xiangya School of Medicine,Central South University

Department of Urology,The Second Hospital,University of South China

⁰