Effects of MMP7 antisense oligodeoxynucleotide on adhesion and invasion of human lung adenocarcinoma cell A549

YANG Bo; et al

doi:10.13406/j.cnki.cyxb.2010.08.031

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Effects of MMP7 antisense oligodeoxynucleotide on adhesion and invasion of human lung adenocarcinoma cell A549

更新时间：2024-01-17

- Effects of MMP7 antisense oligodeoxynucleotide on adhesion and invasion of human lung adenocarcinoma cell A549
- Journal of Chongqing Medical University Vol. 35, Issue 8, Pages: 1160-1163(2010)
- 作者机构：
  
  1. 广州军区武汉总医院肿瘤科
  2. 广州军区武汉总医院干部病房二科
  3. 第三军医大学新桥医院全军肿瘤研究所
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.08.031
  CLC： R734.2
- Published：2010，
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[1]杨波,唐伏秋,高建飞,饶智国,王志新.MMP7反义寡核苷酸对肺腺癌A549细胞粘附和侵袭能力的影响[J].重庆医科大学学报,2010,35(08):1160-1163.

YANG Bo, et al. Effects of MMP7 antisense oligodeoxynucleotide on adhesion and invasion of human lung adenocarcinoma cell A549. [J]. Journal of Chongqing Medical University 35(8):1160-1163(2010)
[1]杨波,唐伏秋,高建飞,饶智国,王志新.MMP7反义寡核苷酸对肺腺癌A549细胞粘附和侵袭能力的影响[J].重庆医科大学学报,2010,35(08):1160-1163. DOI： 10.13406/j.cnki.cyxb.2010.08.031.

YANG Bo, et al. Effects of MMP7 antisense oligodeoxynucleotide on adhesion and invasion of human lung adenocarcinoma cell A549. [J]. Journal of Chongqing Medical University 35(8):1160-1163(2010) DOI： 10.13406/j.cnki.cyxb.2010.08.031.

摘要

目的:观察基质溶解素（Matrilysin

MMP7）反义寡核苷酸（Antisense oligodeoxynucleotide）对肺腺癌A549细胞体外粘附和侵袭能力的影响。方法:采用硫代磷酸修饰的MMP7 ASODN

通过脂质体导入A549细胞

RT-PCR检测MMP7 mRNA表达;平板粘附模型和Boyden chamber模型比较A549细胞的粘附和侵袭能力。结果:MMP7 ASODN转染A549细胞后

与未转染组和脂质体组比较

RT-PCR电泳条带相对光密度值显著降低（P<0.01）。粘附于平板和穿过Boyden chamber的细胞数有明显下降（P<0.01）。结论:MMP7 ASODN可以有效抑制肺腺癌A549细胞株MMP7基因的表达

降低细胞粘附和侵袭能力。

Abstract

Objective:Toinvestigate the influence of MMP7 antisense oligodeoxynucleotide(ASODN)on adhesion and invasion ofhuman lung adenocarcinoma cell line A549.Methods:Phosphorothioate MMP7 ASODN was transfected to A549 cells mediated by liposome. The expression of MMP7 was examined by RT-PCR.The adhesive and invasive ability were examined by plate adhesion model and Boyden Chamber transwell assay.Results:After MMP7 ASODN was transfected

the relative optical density(ROD)of electrophoresis strip was decreased obviously(P<0.01).Compared with A549 group and liposome group

the cells adhering to plate and permeating septum of Boyden chamber were decreased markedly(P<0.01).Conclusion:The MMP7 ASODN could effectively inhibit the expression of MMP7 and reduce the adhesive and invasive ability of A549.

关键词

基质溶解素反义寡核苷酸肺腺癌A549细胞粘附侵袭

Keywords

MatrilysinAntisense oligodeoxynucleotideLung adenocarcinoma cell line A549Adhesioninvasion

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