Endosomatic transference of transplanted bone marrow mesenchymal stem cells in rat of ulcerative colitis

DUAN Zheng; et al

doi:10.13406/j.cnki.cyxb.2010.08.012

您当前的位置：

首页 >

文章列表页 >

Endosomatic transference of transplanted bone marrow mesenchymal stem cells in rat of ulcerative colitis

更新时间：2024-01-17

- Endosomatic transference of transplanted bone marrow mesenchymal stem cells in rat of ulcerative colitis
- Journal of Chongqing Medical University Vol. 35, Issue 8, Pages: 1152-1155(2010)
- 作者机构：
  
  重庆医科大学基础医学院组织胚胎学教研室、干细胞与组织工程研究室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.08.012
  CLC： R574.62
- Published：2010，
扫描看全文
[1]段征,徐艳华,陈小云,汪维伟.间充质干细胞移植后在溃疡性结肠炎大鼠体内的迁移[J].重庆医科大学学报,2010,35(08):1152-1155.

DUAN Zheng, et al. Endosomatic transference of transplanted bone marrow mesenchymal stem cells in rat of ulcerative colitis. [J]. Journal of Chongqing Medical University 35(8):1152-1155(2010)
[1]段征,徐艳华,陈小云,汪维伟.间充质干细胞移植后在溃疡性结肠炎大鼠体内的迁移[J].重庆医科大学学报,2010,35(08):1152-1155. DOI： 10.13406/j.cnki.cyxb.2010.08.012.

DUAN Zheng, et al. Endosomatic transference of transplanted bone marrow mesenchymal stem cells in rat of ulcerative colitis. [J]. Journal of Chongqing Medical University 35(8):1152-1155(2010) DOI： 10.13406/j.cnki.cyxb.2010.08.012.

摘要

目的:观察大鼠骨髓间充质干细胞（Bone marrow mesenc hymal stem cells

BMSCs）静脉移植后在体内的迁移。方法:SD大鼠分为A组[免疫结合TNBS/ethanol诱导溃疡性结肠炎（Ulcerative colitis

UC）模型]和B组（正常大鼠）

均尾静脉注射1ml荧光标记的BMSCs。移植后第3、7、14d各处死5只

取材做恒冷切片

荧光显微镜下观察BMSCs在骨髓、肺、肝、脾以及消化道内分布

测定荧光的积分光密度（Integral optical density

IOD）值。结果:BMSCs移植后多分布于血管丰富器官

7d时肺内荧光IOD值最高

14d时骨髓内IOD值最高

但无统计学意义。14d时

A组结肠的IOD值高于同组其余消化道各段以及B组结肠（P<0.05）。结论:静脉移植的BMSCs早期主要分布在血供丰富的器官中

迁移至受损结肠的BMSCs高于正常结肠。

Abstract

Objective:To investigate the transference of transplanted bone marrow mesenchymal stem cells (BMSCs)in rat of ulcerative colitis. Methods:SD rats were randomly distributed into group A colitis induced by immune-combined TNBS/ethanol and group B as control. All rats received caudal vein injection with 1 ml of fluorescence marked BMSCs

then 5 rats were sacrificed at day 3

7 and 14 respectively in each group. Cryostat sections of bone marrow

lung

liver

spleen and gut were made to observe the distribution of BMSCs in these organs

and the fluorimetric integral optical density(IOD)were analyzed. Results:BMSCs were mainly distributed in organs which richs vessels. The highest IOD level of BMSCs in lung was on the 7th day

and was on the 14th day in bone marrow. On the 14th day

the IOD level of BMSCs in colon of group A were higher than those of other segments of digestive tube and also higher than those in group B (P<0.05). Conclusion:Transplanted BMSCs in earlierperiod were mainly distributed in the organs with rich blood supply

and BMSCs migrated to the injured colon are higherthan those of normal colon.

关键词

骨髓间充质干细胞移植溃疡性结肠炎大鼠

Keywords

bone marrow mesenchymal stem cellstransplantationulcerative colitisrat

references

Views

217

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Distribution of transplanted bone mesenchymal stem cells in colon of ulcerative colitis rat

Sodium nitroprusside promoting the migration of vein transplanted bone marrow mesenchymal stem cells to the colon of ulcerative colitis rat

Comparison of two experimental ulcerative colitis models in rat

Effects of autologous bone marrow mesenchymal stem cells on the renal cell proliferation and apoptosis and renal function in a rabbit of renal ischemia-reperfusion injury

Intervention effect of coix seed polysaccharides on an in vitro inflammation model of ulcerative colitis and its mechanism: a study based on the NLRP3/caspase-1 pathway

Related Author

CHEN Xiao-Yun

et al

姜蓉

汪维伟

CHEN Xiao-yun

et al

姜蓉

汪维伟

Related Institution

Department of Histology and Embryology ,Laboratory of Stem Cells and Tissue Engineering,Chongqing Medical University

Department of Histology and Embryology,Laboratory of Stem Cells and Tissue Engineering,Chongqing Medical University

Department of Histology and Embryology,College of Basic Medicine,Chongqing Medical University

重庆医科大学基础医学院组织胚胎学教研室

Renal Diseases Research Center,Departmentv of Urinary Surgery,Xinqiao Hospital,the Third Military Medical University

⁰