Determination of tetrazanbigen in liver by reversed-phase high performance liquid chromatography

LIU Qiang; et al

doi:10.13406/j.cnki.cyxb.2010.07.049

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Determination of tetrazanbigen in liver by reversed-phase high performance liquid chromatography

更新时间：2024-01-17

- Determination of tetrazanbigen in liver by reversed-phase high performance liquid chromatography
- Journal of Chongqing Medical University Vol. 35, Issue 7, Pages: 1037-1039(2010)
- 作者机构：
  
  重庆医科大学药学院药物化学教研室重庆市生物化学与分子药理学重点实验室重庆市药物工程研究中心
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.07.049
  CLC： R965
- Published：2010，
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[1]刘嫱,陈志琼,靳红卫,李伟,夏铸,程训官,张昕宇,郑小红,余瑜.固相萃取RP-HPLC测定小鼠肝脏组织中的德氮吡格[J].重庆医科大学学报,2010,35(07):1037-1039.

LIU Qiang, et al. Determination of tetrazanbigen in liver by reversed-phase high performance liquid chromatography. [J]. Journal of Chongqing Medical University 35(7):1037-1039(2010)
[1]刘嫱,陈志琼,靳红卫,李伟,夏铸,程训官,张昕宇,郑小红,余瑜.固相萃取RP-HPLC测定小鼠肝脏组织中的德氮吡格[J].重庆医科大学学报,2010,35(07):1037-1039. DOI： 10.13406/j.cnki.cyxb.2010.07.049.

LIU Qiang, et al. Determination of tetrazanbigen in liver by reversed-phase high performance liquid chromatography. [J]. Journal of Chongqing Medical University 35(7):1037-1039(2010) DOI： 10.13406/j.cnki.cyxb.2010.07.049.

摘要

目的:建立小鼠肝脏组织中德氮吡格（Tetrazanbigen）的反相高效液相色谱（RP-HPLC）测定方法。方法:精密称取小鼠肝脏组织

匀浆后固相萃取（Solid phase extraction

SPE）。液相色谱采用Lichrospher C18色谱柱（4.6mm×250mm

5μm）

以甲醇-0.1%三乙胺水溶液（pH6.5）（体积比90∶10）为流动相

流速1.0ml/min

检测波长260nm

进样量20μl。结果:实验表明

肝脏组织匀浆液中德氮吡格在2～20μg/ml范围内与峰面积呈良好的线性关系（r=0.9993）

平均回收率为106.47%。最低定量限为1.0μg/ml。结论:本文建立的固相萃取RP-HPLC方法简便、准确、选择性和重现性好

适合于德氮吡格的肝脏组织浓度检测。

Abstract

Objective:To establish a solid phase extraction(SPE)and reversed-phase high-performance liquid chromatographic (RP-HPLC)method for the determination of tetrazanbigen in liver.Methods:Tetrazanbigen in liver samples was determined after a C18 solid phase extraction on a Lichrospher C18 column at 260 nm with an isocratic mobile phase of 90%methanol in 100 ml methanol -deionized water(pH 6.5)

containing 0.1%triethyl amine.The flow rate was set at 1.0 ml/min.Results:The linearity was good over the concentration range of 2～20μg/ml(r=0.9 993).The average recovery and RSD were 106.47%and 3.0%respectively.The lower limit of quantification was 1.0μg/ml.Conclusion:The method is simple

accurate and can be used to determine the TNBG concentration in liver and study its metabolism.

关键词

德氮吡格固相萃取反相高效液相色谱法肝脏

Keywords

TetrazanbigenSolid phase extractionRP-HPLCliver

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