Apoptosis of mycosis fungoides hut-102 cells induced by triptolide in combination with dexamethasone and its mechanism

ZHANG Bo; et al

doi:10.13406/j.cnki.cyxb.2010.07.042

您当前的位置：

首页 >

文章列表页 >

Apoptosis of mycosis fungoides hut-102 cells induced by triptolide in combination with dexamethasone and its mechanism

更新时间：2024-01-17

- Apoptosis of mycosis fungoides hut-102 cells induced by triptolide in combination with dexamethasone and its mechanism
- Journal of Chongqing Medical University Vol. 35, Issue 7, Pages: 1010-1013(2010)
- 作者机构：
  
  重庆医科大学附属第二医院皮肤科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.07.042
  CLC： R739.5
- Published：2010，
扫描看全文
[1]张波,郝进.雷公藤内酯醇协同地塞米松诱导蕈样肉芽肿hut-102细胞凋亡及其机制[J].重庆医科大学学报,2010,35(07):1010-1013.

ZHANG Bo, et al. Apoptosis of mycosis fungoides hut-102 cells induced by triptolide in combination with dexamethasone and its mechanism. [J]. Journal of Chongqing Medical University 35(7):1010-1013(2010)
[1]张波,郝进.雷公藤内酯醇协同地塞米松诱导蕈样肉芽肿hut-102细胞凋亡及其机制[J].重庆医科大学学报,2010,35(07):1010-1013. DOI： 10.13406/j.cnki.cyxb.2010.07.042.

ZHANG Bo, et al. Apoptosis of mycosis fungoides hut-102 cells induced by triptolide in combination with dexamethasone and its mechanism. [J]. Journal of Chongqing Medical University 35(7):1010-1013(2010) DOI： 10.13406/j.cnki.cyxb.2010.07.042.

摘要

目的:探讨雷公藤内酯醇（Triptolide

TP）与地塞米松联合对蕈样肉芽肿（Mycosis fungoides

MF）hut-102细胞体外增殖、凋亡是否具有协同作用及其机制。方法:MTT法检测细胞存活率;流式细胞术检测细胞凋亡率;蛋白印迹法检测糖皮质激素受体（Glucocorticoid receptor

GR）和凋亡相关蛋白Caspase-3表达。结果:①不同浓度TP（6.25、12.5、25nmol/L）和地塞米松（10-7、10-6、10-5mol/L）对hut-102细胞均有增殖抑制作用

与时间、浓度相关;12.5nmol/LTP与不同浓度地塞米松联合

细胞抑制率随地塞米松浓度升高呈现协同作用。②TP和地塞米松均能诱导hut-102细胞凋亡

联合用药凋亡率明显高于单药

具有交互效应。③10-5mol/L地塞米松作用hut-102细胞后

GR表达降低（P<0.05）

联合用药（12.5nmol/LTP+10-5mol/L地塞米松）GR表达上调、Caspase-3激活增强（P<0.05）。结论:TP联合地塞米松可明显抑制hut-102细胞增殖

增强诱导凋亡

具有协同效应

其作用机制可能与GR表达上调、Caspase-3激活增强有关。

Abstract

Objective:To investigate the effect of triptolide combined with dexamethasone on proliferation and apoptosis of the mycosis fungoides hut-102 cells and the possible mechanisms.Methods:The viability of hut-102 cells was measured by MTT assay;the apoptotic rate of hut-102 cells was detected by flow cytometry.The expression levels of Glucocorticoid receptor(GR)and apoptosis-related protein Caspase-3 were determined by Western blot analysis.Results:①MTT assays showed that different concentrations of triptolide or dexame thasone can effectively inhibit the proliferation of hut-102 cells in a dose and time-dependent manner.When triptolide(12.5 nmol/L)was co-administered with dexamethasone

the inhibition rate increased along with the concentration of dexamethasone.And we observed positive cooperative inhibitory effects of triptolide and dexamethasone on the proliferation of hut-102 cells.②Both triptolide and dexame thasone can independently promote apoptosis of the hut-102 cell.And the apoptotic rate in the combination group was higher than single group(P<0.01).③Compared with the significantly decreased expression of Glucocorticoid receptor(GR)in hut-102 cells in dexameth asone group

the expression of GR and activated caspase-3 increased in the combination group(P<0.05).Conclusion:The apop tosis of hut-102 cells induced by dexamethasone was enhanced by triptolide

which maybe connected with the increased expression of GR and activated Caspase-3 in hut-102 cells.

关键词

蕈样肉芽肿hut-102雷公藤内酯醇地塞米松糖皮质激素受体Caspase-3凋亡

Keywords

Hut-102TriptolidedexamethasoneGlucocorticoid recepto(rGR)Caspase-3apoptosis

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of carthamus tinctorius L.injection on caspase-3,bcl-2 expression related to renal ischemia/reperfusion injury in rats

The investigation of the proliferation inhibition and apoptosis of K562 ninduced by dexamethasone

CCK-8 prevents glutamate-induced apoptosis in cultured cortical neurons via up-regulation of bcl-2/bax ratio and down-regulation of caspase-3

Changes of super microstructure of hippocampus and expressions of Caspase-3 in epileptic rats after injection of YVAD-CHO

Impacts of LncRNA MYLK-AS1 on proliferation，apoptosis，and invasion of gastric cancer cells by regulating the miR-141-3p/STMN1 axis

Related Author

GAO Fei

et al

罗春丽

杨美

LIU Chang

et al

钟梁

刘北忠

Related Institution

Department of Urology,the First Affiliated Hospital,Chongqing Medical University

重庆医科大学检验系

重庆医科大学基础医学院解剖教研室

Key Laboratory of Laboratory Medical Diagnostics of Ministry of Education,Faculty of Laboratory Medicine,Chongqing Medical University

Department of Neurology,the Children’s Hospital,Chongqing MedicaI University

⁰