5-Aza-cytidine inhibit proliferation of human promyelocytic leukemia cells (HL60) by inducing apoptosis

SU Yong-chun; et al

doi:10.13406/j.cnki.cyxb.2010.07.033

您当前的位置：

首页 >

文章列表页 >

5-Aza-cytidine inhibit proliferation of human promyelocytic leukemia cells (HL60) by inducing apoptosis

更新时间：2024-01-17

- 5-Aza-cytidine inhibit proliferation of human promyelocytic leukemia cells (HL60) by inducing apoptosis
- Journal of Chongqing Medical University Vol. 35, Issue 7, Pages: 981-984(2010)
- 作者机构：
  
  重庆医科大学附属儿童医院血液肿瘤科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.07.033
  CLC： R733.7
- Published：2010，
扫描看全文
[1]苏庸春,徐酉华,蔡倩,胡艳妮,李欣,罗庆.甲基化抑制剂5-氮杂胞苷对HL-60细胞增殖及凋亡的影响[J].重庆医科大学学报,2010,35(07):981-984.

SU Yong-chun, et al. 5-Aza-cytidine inhibit proliferation of human promyelocytic leukemia cells (HL60) by inducing apoptosis. [J]. Journal of Chongqing Medical University 35(7):981-984(2010)
[1]苏庸春,徐酉华,蔡倩,胡艳妮,李欣,罗庆.甲基化抑制剂5-氮杂胞苷对HL-60细胞增殖及凋亡的影响[J].重庆医科大学学报,2010,35(07):981-984. DOI： 10.13406/j.cnki.cyxb.2010.07.033.

SU Yong-chun, et al. 5-Aza-cytidine inhibit proliferation of human promyelocytic leukemia cells (HL60) by inducing apoptosis. [J]. Journal of Chongqing Medical University 35(7):981-984(2010) DOI： 10.13406/j.cnki.cyxb.2010.07.033.

摘要

目的:观察5-氮杂胞苷对髓系白血病细胞系HL-60的增殖抑制作用

探讨其对细胞周期、细胞凋亡的影响。方法:常规方法培养HL-60细胞

加入不同浓度的5-氮杂胞苷

以MTT法测定对HL-60的抑制作用

计算细胞生长抑制率;流式细胞术检测小剂量5-氮杂胞苷处理HL-60细胞后48h的细胞凋亡率及细胞周期分布特点。结果:不同浓度5-氮杂胞苷均可以不同程度的抑制细胞生长

且表现为时间、浓度依赖性。在小剂量浓度5-氮杂胞苷处理HL-60细胞48h后

随着5-氮杂胞苷浓度的增加

凋亡细胞百分比逐渐增多（P<0.05）;G1期细胞逐渐增多

S期细胞减少

G2/M期细胞相对增多

细胞阻滞在G1期。结论:5-氮杂胞苷对髓系白细胞细胞系HL-60的生长抑制表现为浓度和时间依赖性

一定剂量的5-氮杂胞苷诱导细胞凋亡

使细胞阻滞在G1期。

Abstract

Objevtive:This study was aimed to investigate the effect of 5-aza-cytidine on the proliferation

cell cycles distribution profile and apoptosis of human promyelocytic cell line HL60.Methods: The human promyelocytic cell line HL-60 was cultured in RPMI 1640 medium.3-(4

5-Dimethylthiazol-2-y)l-2

5-diphenylte trazolium(MTT)reduction by cells was used to assess drug-induced cell growth inhibition.Cells were treated with 0.25

0.5

2.5μmol/L of freshly prepared 5-aza-cytidine for 48 hours before they were harvested for analyzing distribution of cell cycle phases and apoptosis by flow cytometer.Results:5-aza-cytidine inhibited HL60 cell proliferation in a time-and concentration-dependent manner.Apoptosis was induced as detected by flow cytometry.Determination of the cell cycle distribution by flow cytometry revealed an accumulation of cells in G1 phase.Conclusion:DNA methylation inhibitor 5-aza-cytidine has effects on proliferation of human promyelocytic cell line HL60.Possible mechanism of this inhibition is induction of apoptosis.

关键词

基因甲基化5-氮杂胞苷白血病凋亡细胞周期

Keywords

Gene methylation5-aza-CytidineleukemiaCell cycleApoptosis

references

Views

220

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Factors affecting the quality of life in children with leukemia and the relevant research progress at home and abroad

Effect of artesunate on autophagy of leukemic K562 cells and its mechanism

RCIA AND RBC SOD ACTIVITY IN PATIENTS WITH LEUKEMIA AND MALIGNANT LYMPHOMA

DETERMINATION OF ACUTE PROTEIN AND SIALIC ACID IN PATIENTS WITH ACUTE LEUKEMIA

MORPHOLOGICAL AND IMMUNOPHENOTYPICSTUDIES ON ACUTE LEUKEMIA

Related Author

Shi Lin

Mo Lin

He Yun

Liu Yang

Wang Zijuan

Zhang Ping

Yang Guicun

Chen Hongxia

Related Institution

the Third Surgical Ward,the Children’s Hospital,Chongqing Medical University

Special Needs Outpatient,the Children’s Hospital,Chongqing Medical University

Department of Hematology,the Children’s Hospital,Chongqing Medical University,Ministry of Education Key Laboratory of Child Development and Disorders,China International Science andTechnology Cooperation Base of Child Development and Critical Disorders,Chongqing Key Laboratory of Pediatrics

重庆医科大学第一医院内科血液组重庆医科大学检验科

重庆医科大学检验科

⁰