Single nucleotide polymorphisms 936C/T,-460T/C,405G/C in vascular endothelial growth factor gene and their association with cerebral infarction

LI Tian-Lian; et al

doi:10.13406/j.cnki.cyxb.2010.07.028

您当前的位置：

首页 >

文章列表页 >

Single nucleotide polymorphisms 936C/T,-460T/C,405G/C in vascular endothelial growth factor gene and their association with cerebral infarction

更新时间：2024-01-17

- Single nucleotide polymorphisms 936C/T,-460T/C,405G/C in vascular endothelial growth factor gene and their association with cerebral infarction
- Journal of Chongqing Medical University Vol. 35, Issue 7, Pages: 1014-1017(2010)
- 作者机构：
  
  海南省人民医院神经内科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.07.028
  CLC： R743.33
- Published：2010，
扫描看全文
[1]李天炼,廖小平,文国强,邓益东,黄仕雄.血管内皮生长因子936C/T、-460T/C、405G/C单核苷酸多态性与脑梗死的关系[J].重庆医科大学学报,2010,35(07):1014-1017.

LI Tian-Lian, et al. Single nucleotide polymorphisms 936C/T,-460T/C,405G/C in vascular endothelial growth factor gene and their association with cerebral infarction. [J]. Journal of Chongqing Medical University 35(7):1014-1017(2010)
[1]李天炼,廖小平,文国强,邓益东,黄仕雄.血管内皮生长因子936C/T、-460T/C、405G/C单核苷酸多态性与脑梗死的关系[J].重庆医科大学学报,2010,35(07):1014-1017. DOI： 10.13406/j.cnki.cyxb.2010.07.028.

LI Tian-Lian, et al. Single nucleotide polymorphisms 936C/T,-460T/C,405G/C in vascular endothelial growth factor gene and their association with cerebral infarction. [J]. Journal of Chongqing Medical University 35(7):1014-1017(2010) DOI： 10.13406/j.cnki.cyxb.2010.07.028.

摘要

目的:研究血管内皮生长因子（Vascular endothelial growth factor

VEGF）936C/T、-460T/C、405G/C单核苷酸多态性（Single nucleuotide polymorphisms

SNP）与脑梗死（Cerebral infarction

CI）的关系。方法:应用病例对照分析和聚合酶链反应-限制性片段长度多态性方法对150例CI患者与120例健康对照组的VEGF936C/T、-460T/C、405G/C等位基因频率和基因型频率与CI之间的关系进行分析。结果:CI组VEGF936C/T SNP T位点的等位基因频率为0.3667

对照组为0.2500

2组比较有统计学意义（P<0.01）;CI组VEGF第936位点的基因频率（CC:34.00%

CT:58.67%

TT:7.33%）与对照组的基因型频率（CC:55.83%

CT:38.33%

TT:5.83%）差异有统计学意义（P=0.001）

其中CI组CT+TT基因型频率（66.00%）高于对照组（44.17%

P=0.001）;在高血压分层分析后CI组VEGF第936位点的T等位基因频率和CT+TT基因型频率与对照组相比有统计学意义

而两组间-460T/C、405G/C的基因型和等位基因频率的差异无统计学意义。结论:血管内皮生长因子VEGF936C/T位点与CI有关联

可能是CI的独立危险因素

-460T/C、405G/C与CI无关联。

Abstract

Objective:To study the relationship between the single nucleotide polymorphisms (SNP)936C/T

-460T/C

405G/C in Vascular endothelial growth factor (VEGF)gene and their association with cerebral infarction (CI).Methods:Case-control study was carried out to investigate allelic genotypes and frequency of the three VEGF SNP in 150 subjects with CI and 120 control individuals.Polymerase chain reaction-Restriction fragment lengthpolymorphism

(PCR-RFLP)[U3] and DNA sequencing were used to analyze the polymorphisms.The correlations between VEGF SNPs and CI were evaluated.Results:There was a highly significant difference in allele frequency of 936C/T SNP between CI and control subjects

and the T allele frequencies were respectively 0.3667 versus 0.2500(P<0.01).The frequencies of 936C/T SNP genotypes were significantly different(P=0.001)between the CI group(CC:34.00%

CT:58.67%

TT:7.33%)and the control group (CC:55.83%

CT:38.33%

TT:5.83%).The frequency (66.00%)of genotype (CT+TT)of 936C/T SNP in CI group were significantly higher than that (44.17%)in the control group (P=0.001).The frequencies of allele T and genotype (CT+TT)of VEGF936C/T SNP were still associated with CI after stratification of blood pressure.No difference was found in the genotype and allelic frequencies of VEGF-460T/C

405G/C alleles between CI group and the control groups.Conclusion:Our results demonstrated that VEGF936C/T SNP might be associated with CI and be an independent risk factor of CI.But we did not find that VEGF-460T/C

405G/C SNP associated with CI.

关键词

血管内皮生长因子单核苷酸多态性脑梗死

Keywords

vascular endothelial growth factorsingle nucleotide polymorphismscerebral infarction

references

Views

114

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of miR-150 targeting vascular endothelial growth factor on neurological rehabilitation and angiogenesis after cerebral infarction

Effect of remifentanil on the intervertebral disc of rats with intervertebral disc degeneration and its mechanism

Effect of multi-functional hand exercise on patients with hand dysfunction after cerebral infarction

Correlation analysis of serum vascular endothelial growth factor levels and pigment epithelial derived factors and vascular cognitive impairment caused by cerebral small-vascular disease

Serum levels of Cyfra 21.1,galectin-3 and VEGF in patients with differentiated thyroid carcinoma after surgery

Related Author

Guo Dan

Zhang Juan

Wang Hongtao

Liu Xinggao

Guo Yuanjin

Liao Yao

Tu Ting

Zeng Xiyu

Related Institution

1. Department of Rehabilitation Medicine，Union Dongxihu Hospital of Huazhong University of Science and Technology/People’s Hospital of Dongxihu District

Department of Neurology，Union Hospital Affiliated to Tongji Medical College，Huazhong University of Science and Technology

Department of Pain Treatment，Traditional Chinese Medicine Hospital Affiliated to Southwest Medical University

Neurology Department, Shanghai Changzheng Hospital，Naval Medical University

Nursing Department, Shanghai Changzheng Hospital，Naval Medical University

⁰