Temporal regulation of p300 on heart-specific transcription factors during mouse cardiogenesis

YANG Xue-fang; et al

doi:10.13406/j.cnki.cyxb.2010.07.024

您当前的位置：

首页 >

文章列表页 >

Temporal regulation of p300 on heart-specific transcription factors during mouse cardiogenesis

更新时间：2024-01-17

- Temporal regulation of p300 on heart-specific transcription factors during mouse cardiogenesis
- Journal of Chongqing Medical University Vol. 35, Issue 7, Pages: 961-965(2010)
- 作者机构：
  
  1. 重庆医科大学附属儿童医院心脏内科
  2. 重庆医科大学附属儿童医院儿科研究所
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.07.024
  CLC： R725.4
- Published：2010，
扫描看全文
[1]杨雪芳,田杰,陈国珍,孙慧超,钟立霖,吴晓云,朱静.小鼠胚胎心脏发育过程中组蛋白乙酰化酶亚型p300调控心脏特异转录因子的动态表达[J].重庆医科大学学报,2010,35(07):961-965.

YANG Xue-fang, et al. Temporal regulation of p300 on heart-specific transcription factors during mouse cardiogenesis. [J]. Journal of Chongqing Medical University 35(7):961-965(2010)
[1]杨雪芳,田杰,陈国珍,孙慧超,钟立霖,吴晓云,朱静.小鼠胚胎心脏发育过程中组蛋白乙酰化酶亚型p300调控心脏特异转录因子的动态表达[J].重庆医科大学学报,2010,35(07):961-965. DOI： 10.13406/j.cnki.cyxb.2010.07.024.

YANG Xue-fang, et al. Temporal regulation of p300 on heart-specific transcription factors during mouse cardiogenesis. [J]. Journal of Chongqing Medical University 35(7):961-965(2010) DOI： 10.13406/j.cnki.cyxb.2010.07.024.

摘要

目的:观察小鼠胚胎心脏发育过程中GATA4、Nkx2.5、MEF2C、Tbx5基因的动态表达和这些基因的启动子区域组蛋白H3乙酰化修饰状态的变化

以及组蛋白乙酰化酶亚型p300对这些基因启动子的直接调控作用

为进一步研究先天性心脏疾病的发生机制奠定基础。方法:选取胎龄（Embryoday

E）10.5d和16.5d小鼠正常胚胎心脏

用实时荧光定量PCR（RealTimePCR）的方法观察上述基因mRNA的动态表达;采用染色质免疫沉淀技术（Chromatin immunoprecipitation

ChIP）

用ChIP级抗乙酰化H3和抗p300抗体免疫沉淀与其所结合的DNA

采用RealTimePCR扩增抗体所富集的上述基因启动子的DNA量

观察胚胎心脏发育过程中乙酰化组蛋白及组蛋白乙酰化酶亚型p300是否参与调控上述四种心脏特异转录因子的表达。结果:①胎龄E10.5d和E16.5d小鼠胚胎心脏的GATA4、Nkx2.5、MEF2C、Tbx5基因的mRNA表达水平都有明显差异（P<0.05）

其中GATA4和MEF2C mRNA相对β-actin的表达水平

E10.5明显低于E16.5

而Nkx2.5和Tbx5mRNA相对表达水平

E10.5明显高于E16.5;并且ChIP结果显示这些基因启动子的乙酰化水平也随着时间而改变

但差异无统计学意义。②E10.5和E16.5胚胎小鼠心脏中

p300抗体均能免疫沉淀GATA4、Nkx2.5、MEF2C、Tbx5基因启动子

其沉淀的基因量在这两个小鼠心脏发育的关键时间点有所不同

但差异无统计学意义。结论:小鼠胚胎心脏发育过程中

心脏发育相关基因GATA4、Nkx2.5、MEF2C、Tbx5呈现动态表达

提示这些基因在心脏发育不同阶段具有重要作用。ChIP结果提示p300介导的组蛋白乙酰化修饰可能为调节这些基因动态表达的机制之一。

Abstract

Objective:Cardiogenesis is a precise process controlled by sequential gene regulatory steps.Heart-specific transcription factors (TFs)GATA4

Nkx2.5

MEF2C and Tbx5 play critical roles in controlling heart development.But the genetic basis for the temporal expression of genes during heart development still remains unclear.Substantial studies displayed gene regulation partly attributes to histone acetylation.However

the functions of individual histone acetyltransferases (HATs)in specific developmental transcription programs are not well elucidated.p300

a histone acetyltransferase and coactivator

plays key role in many physiological processes

moreover our previous study suggests that expression of p300 is developmentally regulated during mouse cardiogenesis

but the underlying molecular mechanism remains elusive.Methods:The whole hearts from embryonic mice on embryonic days (E)10.5 and 16.5 were collected accordingly

and then total RNA was extracted.Heart-specific TFs GATA4

Nkx2.5

MEF2C and Tbx5 mRNA from mouse myocardium at differential embryonic days during cardiac development were analyzed by quantitative (Q)-PCR.The levels of histone H3 acetylation on these heart-specific TFs promoter and transcription factor binding sites of p300 to these genes were identified by chromatin immunoprecipitation assays (ChIP).Results:The present study furnishes a comparative temporal expression map of cardiac transcriptional factors

during murine heart development (E10.5 and E16.5).The mRNA levels of these genes present dynamic change.On E10.5

the expressions of GATA4 and MEF2C were remarkable lower than those on E16.5 (P<0.01).While Nkx2.5 and Tbx5 gene expression levels were remarkable higher on E10.5(P<0.05).Accordingly

ChIP analysis revealed there exist difference on levels of histoneH3 acetylation of these heart-specific TFs promtor between E10.5 and E16.5.Meanwhile

binding of p300 to GATA4

Nkx2.5

MEF2C and Tbx5 promoter was detected by ChIP assay.Conclusion:Data suggests that heart-specific TFs expression could be regulated by promoter activation through histone H3 acetylation activation

which might be dependent on the cooperation with co-activator p300.These results implicate p300 is an important component of heart development and provides an insight into the mechanisms of the dynamic expression of cardiac transcriptional factors during mouse cardiogenesis.

关键词

p300组蛋白乙酰化转录因子心脏发育染色质免疫沉淀

Keywords

p300histone acetylationTranscription factorsHeart developmentChromatin immunoprecipitation(ChIP)

references

Views

382

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Anacardic acid attenuates myocardial fibrosis through inhibiting histone H4K16ac hyperacetylation mediated by KAT8 in TAC mice

Clinical study on effect of oxiracetam on mild and moderate brain injury.

Expression of P300/CBP-associated factor in hepatocellular carcinoma and its clinicopathological significance

Related Author

Zhang Huanting

Wu Shuqi

Peng Chang

XIE Yanfeng

et al

孙晓川

但炜

唐文渊

Related Institution

Department of Pediatrics，Guizhou Children's Hospital/The Affiliated Hospital of Zunyi Medical University

Department of Neurosurgery,the First Affiliated Hospital,Chongqing Medical University

重庆医科大学附属第一医院神经外科

Department of Gastroenterology，the First Clinical College，Medical College Nanchang University

Department of Elderly Oncology，Jiangxi Provincial Tumor Hospital

⁰