Proteinase-activated receptors regulated the expression of type I/Ⅲ collagen protein of hepatic stellate cell isolated and cultured from SD rats liver

GU Xiao-hong; et al

doi:10.13406/j.cnki.cyxb.2010.07.013

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Proteinase-activated receptors regulated the expression of type I/Ⅲ collagen protein of hepatic stellate cell isolated and cultured from SD rats liver

更新时间：2024-01-17

- Proteinase-activated receptors regulated the expression of type I/Ⅲ collagen protein of hepatic stellate cell isolated and cultured from SD rats liver
- Journal of Chongqing Medical University Vol. 35, Issue 7, Pages: 985-988(2010)
- 作者机构：
  
  1. 第三军医大学大坪医院野战外科研究所VIP健康中心
  2. 第三军医大学大坪医院野战外科研究所神外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.07.013
  CLC： R575.2
- Published：2010，
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[1]顾小红,张云东,刘迎春.PAR_S调控大鼠HSC合成和分泌胶原蛋白的作用[J].重庆医科大学学报,2010,35(07):985-988.

GU Xiao-hong, et al. Proteinase-activated receptors regulated the expression of type I/Ⅲ collagen protein of hepatic stellate cell isolated and cultured from SD rats liver. [J]. Journal of Chongqing Medical University 35(7):985-988(2010)
[1]顾小红,张云东,刘迎春.PAR_S调控大鼠HSC合成和分泌胶原蛋白的作用[J].重庆医科大学学报,2010,35(07):985-988. DOI： 10.13406/j.cnki.cyxb.2010.07.013.

GU Xiao-hong, et al. Proteinase-activated receptors regulated the expression of type I/Ⅲ collagen protein of hepatic stellate cell isolated and cultured from SD rats liver. [J]. Journal of Chongqing Medical University 35(7):985-988(2010) DOI： 10.13406/j.cnki.cyxb.2010.07.013.

摘要

目的:探讨蛋白酶活化受体（Proteinase-activated receptors

PARs）对大鼠肝星状细胞（Hepatic stellate cell

HSC）合成和分泌胶原蛋白的调节作用。方法:体外分离和培养SD大鼠HSC

采用免疫细胞化学和RT-PCR检测PAR1、PAR2、Ⅰ和Ⅲ胶原蛋白的表达及其关系。收集细胞培养上清

并用PARs活性肽和反活性肽刺激培养细胞

检测并观察透明质酸（Hexadecanoi cacid

HA）和层粘连蛋白（Laminin

LA）的水平变化。结果:随着HSC培养时间的延长

培养细胞PAR1、PAR2表达与Ⅰ、Ⅲ胶原蛋白的表达逐渐增多

并呈一致性

14d达高峰。培养上清HA和LN水平也逐渐增高

PARs活性肽刺激培养细胞后

HA和LN表达明显增高。结论:PAR1、PAR2表达与Ⅰ/Ⅲ型胶原蛋白的表达及HA和LA的合成和分泌具有相关性

PARs对HSC的活化、合成和分泌胶原蛋白及肝纤维化的形成可能有促进作用。

Abstract

Objective:To investigate the role of proteinase-activated receptor 1 and 2(PAR1

PARs)in regulation ofthe expression oftype I/Ⅲ collagen protein of hepatic stellate cell isolated and cultured from SD rats liver.Methods:Hepatic stellate cell isolated and cultured from SD rats livers with a modification technique of Friedman and centrifugation of Nycodenz Gradient.Expression of mRNA for PAR1

PAR2

Ⅰcollagen and Ⅲ collagen were examined by reverse transcription polymerase chain reaction(RT-PCR)and protein examined by immunocytochemistry

observated characteristic cell shape

and examined expression of hexadecanoic acid(HA)and laminin(LN)of supernatant from culturesd HSC.Results:In the course of culturing rat HSC

vitamin A autofluorescence and lipid droplet were decreased gradually or lost following HSC activation

the cells morphology changed to stellate cells and cells proliferation activated.Ⅰ/Ⅲ collagen protein expressions were also increased

the expression of HA

PAR1 and PARs paralleled with the expression ofⅠ/Ⅲ collagen protein and correlated with activation and proliferation of HSC.Conclusion:The expression of PARs correlated with activation and proliferation of HSC

could promote the expression ofⅠ/Ⅲ collagen protein

HA and LN.PARs might play an important role in the mechanisms of hepatic fibrosis.

关键词

蛋白酶活化受体肝星状细胞胶原蛋白肝纤维化

Keywords

Protease activated receptorHSCCollagenliver fibrosis

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