p75NTR expression in cultured hippocampal neurons during glutamate-induced excitotoxicity

WU Lan-xiang; et al

doi:10.13406/j.cnki.cyxb.2010.07.006

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p75NTR expression in cultured hippocampal neurons during glutamate-induced excitotoxicity

更新时间：2024-01-17

- p75NTR expression in cultured hippocampal neurons during glutamate-induced excitotoxicity
- Journal of Chongqing Medical University Vol. 35, Issue 7, Pages: 992-995(2010)
- 作者机构：
  
  重庆医科大学生命科学研究院
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.07.006
  CLC： R363
- Published：2010，
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[1]吴兰香,滕永真,李春莉,吴明军.p75NTR在谷氨酸诱导海马神经元兴奋毒性损伤中的表达[J].重庆医科大学学报,2010,35(07):992-995.

WU Lan-xiang, et al. p75NTR expression in cultured hippocampal neurons during glutamate-induced excitotoxicity. [J]. Journal of Chongqing Medical University 35(7):992-995(2010)
[1]吴兰香,滕永真,李春莉,吴明军.p75NTR在谷氨酸诱导海马神经元兴奋毒性损伤中的表达[J].重庆医科大学学报,2010,35(07):992-995. DOI： 10.13406/j.cnki.cyxb.2010.07.006.

WU Lan-xiang, et al. p75NTR expression in cultured hippocampal neurons during glutamate-induced excitotoxicity. [J]. Journal of Chongqing Medical University 35(7):992-995(2010) DOI： 10.13406/j.cnki.cyxb.2010.07.006.

摘要

目的:观察谷氨酸诱导海马神经元兴奋毒性损伤中神经营养因子受体p75（p75 neurotrophin receptor

p75NTR）表达水平的变化。方法:建立谷氨酸诱导的新生大鼠海马神经元兴奋毒性损伤模型

RT-PCR和Western blot方法检测p75NTR在mRNA和蛋白质水平的表达变化。结果:谷氨酸刺激后

p75NTR在mRNA（P<0.01）和蛋白质（P<0.05）水平的表达较对照组明显增高;N-甲基-D-门冬氨酸受体（N-methyl-D-aspartate receptor

NMDAR）的特异性拮抗剂MK-801可明显减弱p75NTR在mRNA和蛋白质水平的表达上调（P<0.05）。结论:p75NTR的活化与谷氨酸兴奋毒性神经损伤过程密切相关。

Abstract

Objective:To observe the change in expression level ofp75NTR(p75 neurotrophin receptor)in cultured hippocampal neurons during glutamate-induced excitotoxicity.Methods:Excitotoxicity was induced by glutamate in rat cultured hippocampal neurons; RT-PCR and western blot were used to detect the change in expression level of p75NTR.Results:After glutamate exposure

p75NTR mRNA(P<0.01)and protein(P<0.05)levels had evaluated markedly compared with control group;MK-801

a specific antagonist of N-methyl-D-aspartate recepto(rNMDAR)could decrease p75NTR mRNA and protein levels dramatically compared with glutamate group (P<0.05).Conclusion:p75NTR plays an important role in neuronal damage during glutamate-induced excitotoxicity.

关键词

谷氨酸兴奋毒性p75NTR

Keywords

glutamateExcitotoxicityp75NTR

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