Effects of Fuyuan capsule on expression of IL-1β,MMP-13 and iNOS in synovium in experimental osteoarthritis of rabbits

WU Heng-ke; et al

doi:10.13406/j.cnki.cyxb.2010.07.003

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Effects of Fuyuan capsule on expression of IL-1β,MMP-13 and iNOS in synovium in experimental osteoarthritis of rabbits

更新时间：2024-01-17

- Effects of Fuyuan capsule on expression of IL-1β,MMP-13 and iNOS in synovium in experimental osteoarthritis of rabbits
- Journal of Chongqing Medical University Vol. 35, Issue 7, Pages: 1004-1009(2010)
- 作者机构：
  
  重庆医科大学附属第一医院中西医结合科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.07.003
  CLC： R285.5
- Published：2010，
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[1]巫桁锞,李荣亨,钟玉.复元胶囊对兔膝骨关节炎滑膜IL-1β、MMP-13及iNOS表达的影响[J].重庆医科大学学报,2010,35(07):1004-1009.

WU Heng-ke, et al. Effects of Fuyuan capsule on expression of IL-1β,MMP-13 and iNOS in synovium in experimental osteoarthritis of rabbits. [J]. Journal of Chongqing Medical University 35(7):1004-1009(2010)
[1]巫桁锞,李荣亨,钟玉.复元胶囊对兔膝骨关节炎滑膜IL-1β、MMP-13及iNOS表达的影响[J].重庆医科大学学报,2010,35(07):1004-1009. DOI： 10.13406/j.cnki.cyxb.2010.07.003.

WU Heng-ke, et al. Effects of Fuyuan capsule on expression of IL-1β,MMP-13 and iNOS in synovium in experimental osteoarthritis of rabbits. [J]. Journal of Chongqing Medical University 35(7):1004-1009(2010) DOI： 10.13406/j.cnki.cyxb.2010.07.003.

摘要

目的:通过观察复元胶囊对实验性兔膝骨关节炎（Osteoarthritis

OA）滑膜组织形态学变化的影响

以及复元胶囊对滑膜白细胞介素-1β（Interleukin-1 β）、基质金属蛋白酶-13（Matrix metallo proteinase-13）及诱导型一氧化氮合酶（Induced nitric oxide synthase）表达的影响

探讨具有补益肝肾、益气活血作用的复元胶囊对实验性兔膝OA的治疗作用及其机理。方法:60只新西兰兔

随机分为复元胶囊低、中、高剂量组、模型组和正常组

用改良Hulth法制造兔膝OA模型

中药组给予复元胶囊灌胃

模型组给予生理盐水灌胃

连续分别用药4、8周和12周。HE染色光镜观察滑膜组织形态并评分

透射电镜观察滑膜组织超微结构

免疫组化检测滑膜IL-1β、MMP-13及iNOS的表达。实验后对滑膜评分和3个指标（IL-1β、MMP-13、iNOS）进行前后比较和组间比较。结果:复元胶囊低、中、高剂量组3个时间段的滑膜炎症明显小于同期模型组（P<0.05）

这3个治疗组3个时间段的滑膜IL-1β、MMP-13及iNOS的表达显著低于同期模型组（P<0.05）

中、高剂量组的差异非常显著（P<0.01）。结论:复元胶囊可明显改善实验性兔膝OA滑膜组织形态学的病理改变

显著抑制实验性兔膝OA滑膜组织的IL-1β、MMP-13和iNOS的表达。复元胶囊可改善实验性兔膝OA滑膜组织形态学的病理改变的作用与其抑制滑膜组织IL-1β、MMP-13和iNOS的表达密切相关。

Abstract

Objective:To observe the effects of fuyuan capsule on change of morphology of synovium

expression of IL-1β、MMP-13 and iNOS in synovium in experimental osteoarthritis of rabbits

and investigate the mechanism about the reparation and treatment of fuyuan capsule.Methods:Sixty New Zealand rabbits were divided into five groups randomly:normol group

model contrast group

low dose group

middle dose group and high dose group of fuyuan capsule.OA model was induced in rabbits by improved Hulth’S method.Three Chinese herb groups were treated with fuyuan capsule(0.727

1.453

4.795 g(/kg·d))

continuously for 4

8 weeks and 12 weeks

model contrast group was treated with 0.9%NS

at the same time.Synovium sections were analyzed by immunohistochemistry for the IL-1β

MMP-13 and iNOS

observation and measurement of the change of morphology of synovium by light microscopic and transmission electron microscopic.After the experiment

the differences of these indexes between Three Chinese herb groups and model contrast group were compared.Results:There are found significant differences about measurement of morphology of synovium between Three Chinese herb groups and model contrast group after the experiment(P<0.05)

especially high and middle dosage of fuyuan capsule group(P<0.01).It is the same as the comparison of the expression of IL-1β

MMP-13 and iNOS.Conclusion:Fuyuan capsule can improve the pathological change of morphology of synovium

and decrease the expression of IL-1β

MMP-13 and iNOS in synovium in experimental osteoarthritis of rabbit.There was therapeutical effect on synovium in experimental osteoarthritis of rabbit.

关键词

骨关节炎滑膜复元胶囊白细胞介素-1β诱导型一氧化氮合酶基质金属蛋白酶-13

Keywords

osteoarthritisSynoviumFuyuan capsuleInterleukin-1 βInduced nitric oxide synthaseMatrix metallo proteinase-13

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