Effects of osteoblastic growth peptide on the proliferation in human periodontal ligament cells located on titanium disc in vitro.

ZHOU Wen-wen; et al

doi:10.13406/j.cnki.cyxb.2010.06.044

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Effects of osteoblastic growth peptide on the proliferation in human periodontal ligament cells located on titanium disc in vitro.

更新时间：2024-01-17

- Effects of osteoblastic growth peptide on the proliferation in human periodontal ligament cells located on titanium disc in vitro.
- Journal of Chongqing Medical University Vol. 35, Issue 6, Pages: 873-876(2010)
- 作者机构：
  
  重庆医科大学附属口腔医院修复科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.06.044
  CLC： R780.2
- Published：2010，
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[1]周雯雯,王璐,徐凌.成骨生长肽对钛表面人牙周膜成纤维细胞增殖分化影响的研究[J].重庆医科大学学报,2010,35(06):873-876.

ZHOU Wen-wen, et al. Effects of osteoblastic growth peptide on the proliferation in human periodontal ligament cells located on titanium disc in vitro.. [J]. Journal of Chongqing Medical University 35(6):873-876(2010)
[1]周雯雯,王璐,徐凌.成骨生长肽对钛表面人牙周膜成纤维细胞增殖分化影响的研究[J].重庆医科大学学报,2010,35(06):873-876. DOI： 10.13406/j.cnki.cyxb.2010.06.044.

ZHOU Wen-wen, et al. Effects of osteoblastic growth peptide on the proliferation in human periodontal ligament cells located on titanium disc in vitro.. [J]. Journal of Chongqing Medical University 35(6):873-876(2010) DOI： 10.13406/j.cnki.cyxb.2010.06.044.

摘要

目的:研究成骨生长肽（Osteogenic growth peptide

OGP）对人牙周膜成纤维细胞（Periodontal ligament cells

PDLCs）在钛金属表面增殖分化的影响。方法:将纯钛试件放在12孔培养板内

取生长良好的第五代人牙周膜成纤维细胞接种在试件表面

加入不同浓度的OGP（10-11～10-7mol/L）

分别在接种后1、3、5、7d应用MTT法检测细胞增殖;接种后3、7、10d应用酶联免疫法检测细胞内碱性磷酸酶（Alkaline phosphatase

ALP）活性的表达。结果:OGP在该浓度范围内时

对钛表面的人牙周膜成纤维细胞的增殖率有明显的促进作用（P<0.05）

并且能促进细胞内ALP表达活性（P<0.05）

最佳作用浓度为10-9mol/L。结论:OGP可促进钛片表面人牙周膜成纤维细胞的增殖活性

同时可以增强细胞碱性磷酸酶表达的活性

提示OGP在口腔种植领域中具有良好的应用前景。

Abstract

Objective:To elucidate the effect of osteogenic growth peptide (OGP) on proliferation and differentiation of human periodontal ligament cells on titanium disc in vitro. Methods:Human periodontal ligament cells were cultured located on titanium disc individually in medium contained various concentrations of OGP;and cell proliferation quantity were examined by MTT and alkaline phosphatase activity were examined by enzyme-linked immunosorbentassay. Results:In vitro

the addition of OGP in cultures resulted in an increase of cell proliferation and ALP activity(P<0.05). All OGP concentrations used (10-11~10-7mol/L)were effective but the maximum response was obtained with 10-9mol/L OGP. Conclusion:Our results demonstrate that OGP stimulates proliferation and ALP expression of human periodontal ligament cells located on titanium disc in vitro and suggest that OGP is a promising growth factor in the region of oral implant.

关键词

成骨生长肽人牙周膜成纤维细胞细胞增殖碱性磷酸酶

Keywords

Osteogenic growth peptideHuman periodontal ligament cellsproliferationALP

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