Objective: design knee prostheses infection modal for rabbits. Methods:The knee implants were designed with 3mm To Kirschner wire for the length of 15mm.Implanted the stem to the distal femoral through intercondylar fossa.forty eight rabbits were divided into 4 groups randomly

12 in each group and finished above-mentioned surgery. Immediately after the surgery

0.5 ml 1×107 CFU of Staphylococcus aureus ATCC 25923 was injected into the joints of group A

0.5 ml 1×106 CFU in group B and 0.5 ml 1×105 CFU in group C

group D were not injected.the animals were sacrificed 2 weeks after injections

wounds were observed and X-ray films were and taken. levels of serum CRP and ESR determined

pathological changes were observed and bacteria cultures were performed. Results: After staphylococcus aureus was injected into the joints right after the surgery.100% knee joint infection in group A and 100%in group B

83.3% in group C

none knee joint infection in group D. A large amount of inflammatory cells were found in the infected samples

theand levels of serum CRP and ESR were elevated in the infected samples. Conclusion:An animal model of TKA infection can be established successfully at 2 weeks by 0.5 ml 1×106 CFU staphylococcus aureus ATCC25923 injecting into the joints immediately after the surgery.