Influence of different glucose concentrations on the apoptosis of MC3T3-E1 osteoblast

FENG Zheng-ping; et al

doi:10.13406/j.cnki.cyxb.2010.06.021

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Influence of different glucose concentrations on the apoptosis of MC3T3-E1 osteoblast

更新时间：2024-01-17

- Influence of different glucose concentrations on the apoptosis of MC3T3-E1 osteoblast
- Journal of Chongqing Medical University Vol. 35, Issue 6, Pages: 840-842(2010)
- 作者机构：
  
  1. 重庆医科大学附属第一医院内分泌科
  2. 重庆医科大学干细胞与组织工程实验室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.06.021
  CLC： R580;R587.1
- Published：2010，
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[1]冯正平,邓华聪,杜佳,陈丹燕,姜蓉.不同浓度葡萄糖对成骨细胞凋亡的影响[J].重庆医科大学学报,2010,35(06):840-842.

FENG Zheng-ping, et al. Influence of different glucose concentrations on the apoptosis of MC3T3-E1 osteoblast. [J]. Journal of Chongqing Medical University 35(6):840-842(2010)
[1]冯正平,邓华聪,杜佳,陈丹燕,姜蓉.不同浓度葡萄糖对成骨细胞凋亡的影响[J].重庆医科大学学报,2010,35(06):840-842. DOI： 10.13406/j.cnki.cyxb.2010.06.021.

FENG Zheng-ping, et al. Influence of different glucose concentrations on the apoptosis of MC3T3-E1 osteoblast. [J]. Journal of Chongqing Medical University 35(6):840-842(2010) DOI： 10.13406/j.cnki.cyxb.2010.06.021.

摘要

目的:观察不同浓度葡萄糖对体外培养的MC3T3-E1成骨细胞凋亡的影响。方法:不同浓度的葡萄糖、甘露醇分别刺激体外培养的MC3T3-E1成骨细胞1、3、7、12d

用流式细胞术检测成骨细胞的凋亡。结果:甘露醇组MC3T3-E1成骨细胞凋亡较正常对照组有一定的增加

但无统计学差异（P>0.05）;而葡萄糖呈浓度、时间依赖性诱导MC3T3-E1成骨细胞凋亡（P<0.05

P<0.01）。结论:高糖状态下

MC3T3-E1成骨细胞凋亡显著增加。

Abstract

Objective:To study the influence of different glucose concentrations on the apoptosis of MC3T3-E1 osteoblast. Methods: Seven culture media of different glucose and mannitol concontrations

5.5

11.1

22.2

33.3 mmol/L were set up. MC3T3-E1 osteolblast was cultured in these media successively for 1

12 d

and then the percentages of apoptotic cells were measured by flow cytometry. Results:High mannitol can slightly increase the apoptosis of MC3T3-E1 osteoblast

but there was no statistical significance(P>0.05). The inducing action of glucose to the apoptsis of MC3T3-E1 osteoblast was in concentration and time dependent manner(P<0.05

P<0.01). Conclusion:Glucose of elevated concentration significantly promotes the apoptosis of MC3T3-E1 osteoblast.

关键词

葡萄糖成骨细胞细胞凋亡

Keywords

glucoseosteoblastapoptosis

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