Effect of ATRA on recruiting of MSCs and MMP-9 mRNA expression in damage tissue

YANG Wei; et al

doi:10.13406/j.cnki.cyxb.2010.06.019

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Effect of ATRA on recruiting of MSCs and MMP-9 mRNA expression in damage tissue

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- Effect of ATRA on recruiting of MSCs and MMP-9 mRNA expression in damage tissue
- Journal of Chongqing Medical University Vol. 35, Issue 6, Pages: 869-872(2010)
- 作者机构：
  
  重庆医科大学附属第一医院心胸外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.06.019
  CLC： R563.9
- Published：2010，
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[1]杨威,温剑虎.ATRA诱导下MSCs在损伤肺组织的招募及对MMP-9mRNA的影响[J].重庆医科大学学报,2010,35(06):869-872.

YANG Wei, et al. Effect of ATRA on recruiting of MSCs and MMP-9 mRNA expression in damage tissue. [J]. Journal of Chongqing Medical University 35(6):869-872(2010)
[1]杨威,温剑虎.ATRA诱导下MSCs在损伤肺组织的招募及对MMP-9mRNA的影响[J].重庆医科大学学报,2010,35(06):869-872. DOI： 10.13406/j.cnki.cyxb.2010.06.019.

YANG Wei, et al. Effect of ATRA on recruiting of MSCs and MMP-9 mRNA expression in damage tissue. [J]. Journal of Chongqing Medical University 35(6):869-872(2010) DOI： 10.13406/j.cnki.cyxb.2010.06.019.

摘要

目的:对全反式维甲酸（All-trans retinoic acid

ATRA）诱导骨髓间充质干细胞（Mesenchymal stemcells

MSCs）在损伤肺组织的招募情况同组织中基质金属蛋白酶9信使核糖核酸（Matrix metalloproteinase 9 mRNA

MMP-9 mRNA）的表达情况进行相关性的研究。方法:对实验动物随机给予MSCs及ATRA的方式

分别于2、3、4周后取肺组织切片

激光共聚焦显微镜下观察植入细胞的招募情况

RT-PCR法检测组织中MMP-9 mRNA的表达变化。结果:随着时间的延长

在各时间点:在实验组

可见较多经过标记后的MSCs分布于肺泡表面

与MSCs移植组

ATRA干预组及病例对照组相比较其积分光密度（Integral optical density

IOD）明显增强

差异有统计学意义（P<0.05）。同时MMP-9 mRNA在病例对照组及ATRA干预组较高水平表达

而MSCs移植组中随着MSCs移植后时间延长

其表达水平随之降低

比较差异有统计学意义（P<0.05）

而实验组MMP-9 mRNA在2、3、4周表达又较MSCs移植组低

两者比较差异有统计学意义（P<0.05）。结论:ATRA对肺损伤组织中移植MSCs的招募作用的影响与组织中MMP-9 mRNA水平的降低有关。

Abstract

Objective:Toresearch the correlation between the effect of all-trans retinoic acid (ATRA) on recruitingMSCs and MMP-9 mRNA expression in damage tissue. Methods:MSCs and ATRA were separately used in experimental SD rats.At 2 weeks

3 weeks and 4 weeks after the treatment

RT-PCR assay was used to determine expressions of MMP-9 mRNA in lung tissues.Laser scanning microscope was used to observe the recruitment of implanted MSCs.Results:We found that

at each time point

experimental group had more implanted MSCs in the surface of alveoli and increased IOD than other groups.There was significant difference in these groups (P<0.05). At the same time

in control group and ATRA intervention group

there were overexpression of MMP-9 mRNA

but in MSCs implanted group the expression of MMP-9 mRNA decreased slowly

as compared with the above two groups (P<0.05).In experimental group

the drop of MMP-9 mRNA expression was clear

as compared with the above three groups (P<0.05).Conclusion:The effect of ATRA on recruitiment of MSCs had relationship with the drop of MMP-9 mRNA expression in damage tissue.

关键词

骨髓间充质干细胞肺损伤招募基质金属蛋白酶9

Keywords

MSCslung injuryRecruitementMMP-9

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