The study on gene silence of STAT6 gene by RNA interference

LIU Dan-dan; et al

doi:10.13406/j.cnki.cyxb.2010.06.009

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The study on gene silence of STAT6 gene by RNA interference

更新时间：2024-01-17

- The study on gene silence of STAT6 gene by RNA interference
- Journal of Chongqing Medical University Vol. 35, Issue 6, Pages: 881-884(2010)
- 作者机构：
  
  重庆医科大学附属儿童医院呼吸科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.06.009
  CLC： R346
- Published：2010，
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[1]刘丹丹,符州,田代印,杨琴,王莉佳.RNA干扰抑制STAT6基因表达的初步研究[J].重庆医科大学学报,2010,35(06):881-884.

LIU Dan-dan, et al. The study on gene silence of STAT6 gene by RNA interference. [J]. Journal of Chongqing Medical University 35(6):881-884(2010)
[1]刘丹丹,符州,田代印,杨琴,王莉佳.RNA干扰抑制STAT6基因表达的初步研究[J].重庆医科大学学报,2010,35(06):881-884. DOI： 10.13406/j.cnki.cyxb.2010.06.009.

LIU Dan-dan, et al. The study on gene silence of STAT6 gene by RNA interference. [J]. Journal of Chongqing Medical University 35(6):881-884(2010) DOI： 10.13406/j.cnki.cyxb.2010.06.009.

摘要

目的:构建针对信号转导子和转录激活子6（Singnal transducers and activators of transcription 6

STAT6）基因的干扰质粒

并观察其对STAT6蛋白表达的抑制效果。方法:将构建的短发夹干扰质粒（Short hairpin RNA

shRNA）表达载体

与合成的pIRES2-EGFP-STAT6基因表达质粒共同转染COS7细胞

荧光显微镜下观察干扰效果

并利用Western blot观察蛋白表达的变化。结果:将pIRES2-EGFP-STAT6重组载体转染COS7细胞后

结果显示STAT6蛋白可以在细胞中高水平表达;与阴性对照组相比

STAT6 shRNA-1干扰组和STAT6 shRNA-2干扰组STAT6蛋白表达水平分别是23.1%和9.6%。结论:成功构建并筛选到具有显著干扰效率的STAT6干扰质粒

为哮喘的基因治疗研究奠定了基础。

Abstract

Objective:To construct the recombinant plasmid expressing STAT6 shRNA and observe the effects of RNAi suppressed STAT6 gene expression. Methods:shRNA and pIRES2-EGFP-STAT6 plasmid were cotransfected into COS7 cell

to investigate the suppression effect of shRNA on STAT6 under fluorescent microscope . This effect was confirmed by Western blot at protein expression level. Results: STAT6 protein exhibitited high expression in COS7 cells after transfecting pIRES2-EGFP-STAT6 plasmid into COS7 cells alone ; compare with the negative group

the level of the STAT6 protein in the two shRNAs group were 23.1% and 9.6%. Conclusion:STAT6 shRNA were successfully constructed.This technique deserves further study as a potential therapentic tool for asthma .

关键词

STAT6基因STAT6蛋白RNA干扰（RNAinterferenceRNAi）shRNA哮喘

Keywords

STAT6 geneSTAT6 proteinRNAishRNAasthma

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Related Institution

Department of Respiratory,the Children’s Hospital,Chongqing Medical University

Department of Respiratory Medicine，Children’s Hospital，Zhejiang University School of Medicine/ National Clinical Research Center for Child Health

Department of Pediatrics，The Affiliated Hospital of Nanjing University of Chinese Medicine，The First Clinical Medical College of Nanjing University of Chinese Medicine

Department of Traditional Chinese Medicine，Children’s Hospital，Zhejiang University School of Medicine/ National Clinical Research Center for Child Health

Department of Emergency and Critical Care Medicine,The First Affiliated Hospital of Chongqing Medical University

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