DNA microarray screening and analysis of the virulence gene in golden hamster cheek poch mucosa with precancerous lesions

ZHANG Guo-dong; et al

doi:10.13406/j.cnki.cyxb.2010.05.043

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DNA microarray screening and analysis of the virulence gene in golden hamster cheek poch mucosa with precancerous lesions

更新时间：2024-01-17

- DNA microarray screening and analysis of the virulence gene in golden hamster cheek poch mucosa with precancerous lesions
- Journal of Chongqing Medical University Vol. 35, Issue 5, Pages: 706-710(2010)
- 作者机构：
  
  重庆医科大学附属第一医院口腔颌面外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.05.043
  CLC： R739.8
- Published：2010，
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[1]张国栋,杨凯,梅杰.用全基因芯片技术对金黄地鼠口腔颊黏膜癌前病变相关基因的筛选和分析[J].重庆医科大学学报,2010,35(05):706-710.

ZHANG Guo-dong, et al. DNA microarray screening and analysis of the virulence gene in golden hamster cheek poch mucosa with precancerous lesions. [J]. Journal of Chongqing Medical University 35(5):706-710(2010)
[1]张国栋,杨凯,梅杰.用全基因芯片技术对金黄地鼠口腔颊黏膜癌前病变相关基因的筛选和分析[J].重庆医科大学学报,2010,35(05):706-710. DOI： 10.13406/j.cnki.cyxb.2010.05.043.

ZHANG Guo-dong, et al. DNA microarray screening and analysis of the virulence gene in golden hamster cheek poch mucosa with precancerous lesions. [J]. Journal of Chongqing Medical University 35(5):706-710(2010) DOI： 10.13406/j.cnki.cyxb.2010.05.043.

摘要

目的:利用全基因芯片技术筛选口腔颊黏膜癌前病变发生发展的相关致病基因。方法:用9、10-二甲基;1

2-苯并蒽（Dimethyl benzanthracene

DMBA）诱导建立金黄地鼠颊鳞癌模型

分别提取正常颊黏膜和癌前病变组织的总RNA并合成用Cy3荧光标记的cRNA

分别与含有41000个基因/ESTs序列的Agilent大鼠全基因芯片杂交

以Log2Ratio≥2.5和Log2Ratio≤-2.5为阈值来确定差异表达基因

然后用Gene Ontology对差异表达基因行功能分类分析

最后用RT-PCR对部分差异表达基因进行验证。结果:金黄地鼠颊黏膜自正常黏膜到癌前病变发展过程中

共得到1331条差异表达基因

其中上调基因747条

下调基因584条。Gene Ontology分析发现这些差异基因主要涉及到大分子代谢、信号传导等8大功能分类。对上调基因Atp6v0d2和下调基因Sfrp2行RT-PCR验证结果与芯片结果相符。结论:口腔颊黏膜癌前病变的发生涉及众多基因表达的改变

通过对这些基因的进一步研究

将对探索口腔黏膜癌前病变的发病机制、有效的干预防止癌前病变的发生或逆转癌前病变具有重要意义。

Abstract

Objective:The aim of this study was to screen and analysize the important virulent genes in golden hamster cheek pochmucosa with precancerous lesions induced by DMBA.Methods:The models of golden hamster cheek poch mucosa precancerous lesions were induced by DMBA(styrene-dimethyl-anthracene).We extracted the total RNA from the normal mucosa and precancerous lesions;then we respectively used the Cy3 to mark the cDNA of normal mucosa and precancerous lesions;and used the Agilent Rat microarray containing 41

000 genes/ESTs to screen the differentially expressed genes;used the screening criteria of Log2Ratio ≥2.5 and Log2Ratio≤-2.5

at last using the Gene Ontology to analysis the biology function of the important virulent genes.Meanwhile

we confirmed the correctness of the results of the Gene Chip by using the RT-PCR to verificate some important pathogenic genes.Results:We detected a total of 1331 differentially expressed genes(up-regulated 747;down-regulated 584).GO analysis showed that these differentially expressed genes mainly related to the macromolecular metabolism

signal transduction and so on.The RT-PCR results of Atp6v0d2 and Sfrp2 were consistent with the gene chip analysis.Conclusion:Towards the screening and analysis of the important differentlily expressed genes in the gold hamster cheek pouch mucosa with precancerous lesions

we found that the special stage-precancerous lesions involved in changes in multiple genes.The further study of these genes is useful to explore the pathogenesis of precancerous lesions in the oral mucosa and effectively prevent the occurrence of precancerous lesions.

关键词

颊囊黏膜癌前病变全基因表达谱基因芯片

Keywords

Cheek poch mucosaprecancerous lesionsGene expression profilinggene chip

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