Expression and significance of ABCG2 in U251cells and U251 stem cells

ZHAO Hong-xin; et al

doi:10.13406/j.cnki.cyxb.2010.05.042

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Expression and significance of ABCG2 in U251cells and U251 stem cells

更新时间：2024-01-17

- Expression and significance of ABCG2 in U251cells and U251 stem cells
- Journal of Chongqing Medical University Vol. 35, Issue 5, Pages: 641-644(2010)
- 作者机构：
  
  重庆医科大学附属第二医院神经外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.05.042
  CLC： R739.41
- Published：2010，
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[1]赵洪新,程远,陈松.ABCG2在U251细胞株及其干细胞中的表达及意义[J].重庆医科大学学报,2010,35(05):641-644.

ZHAO Hong-xin, et al. Expression and significance of ABCG2 in U251cells and U251 stem cells. [J]. Journal of Chongqing Medical University 35(5):641-644(2010)
[1]赵洪新,程远,陈松.ABCG2在U251细胞株及其干细胞中的表达及意义[J].重庆医科大学学报,2010,35(05):641-644. DOI： 10.13406/j.cnki.cyxb.2010.05.042.

ZHAO Hong-xin, et al. Expression and significance of ABCG2 in U251cells and U251 stem cells. [J]. Journal of Chongqing Medical University 35(5):641-644(2010) DOI： 10.13406/j.cnki.cyxb.2010.05.042.

摘要

目的:研究ATP转运体成员ABCG2在U251细胞和U251干细胞中的表达

并分析其意义。方法:采用含EGF、bFGF、LIF及B27的无血清培养基培养U251细胞

形成U251干细胞球后采用单细胞克隆法继续在无血清培养基中培养。应用细胞免疫荧光染色法对肿瘤干细胞进行鉴定

应用激光共聚焦和Western blot法检测ABCG2在U251细胞及其干细胞中的表达情况。结果:在U251细胞株中成功分离出U251干细胞;免疫荧光染色显示U251干细胞表达CD133;激光共聚焦显示两种细胞ABCG2均阳性表达

主要位于胞膜;Western blot显示ABCG2在两种细胞均有表达

U251干细胞中ABCG2表达明显高于U251细胞

二者差异有统计学意义（P<0.05）。结论:ABCG2在U251细胞株及U251干细胞中均有表达

以U251干细胞中表达量更多。

Abstract

Objective:To investigate the expression and significance of ABCG2(the G2 member of ATP-binding cassette family)in U251and its stem cell.Methods:The U251 were cultured in serum-free medium with EGF

bFGF

LIF and B27.The tumor spheres formed and cultured continuously in the serum-free medium through the monoclonal formation experiment.The immunofluorescence staining was employed to identify the U251 stem cell.The expression of ABCG2 was detected by confocal microscopy and Western blot.Results:The stem cells from malignant glioma cell line U251 have been successfully isolated.The cells expressed CD 133.The ABCG2 protein positive stain was located in the cell membrane in both U251 cells and its stem cell with confocal microscopy method.Western blotting showABCG2 was expressed in U251 cells and in its stem cells.The expression level in the stem cells was significantly higher than U251 cells.Conclusion:ABCG2 is expressed in U251 cells and in U251 stem cells.The expression level in the latter was higher.

关键词

胶质瘤胶质瘤干细胞ABCG2

Keywords

gliomaGlioma stem cellABCG2

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重庆医科大学临床学院神经外科

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