Application of <sup>99m</sup>Tc-EGFR-McAb to radioimmunoimaging for nude mice bearing human lung adenocarcinoma

DUAN Dong; et al

doi:10.13406/j.cnki.cyxb.2010.05.039

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Application of ^99mTc-EGFR-McAb to radioimmunoimaging for nude mice bearing human lung adenocarcinoma

更新时间：2024-01-17

- Application of ^99mTc-EGFR-McAb to radioimmunoimaging for nude mice bearing human lung adenocarcinoma
- Journal of Chongqing Medical University Vol. 35, Issue 5, Pages: 695-699(2010)
- 作者机构：
  
  1. 重庆医科大学附属第一医院核医学科
  2. 重庆医科大学基础医学院核医学教研室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.05.039
  CLC： R734.2
- Published：2010，
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[1]段东,朱玉泉,李少林.~(99m)Tc-EGFR-McAb用于荷人肺腺癌裸鼠的放射免疫显像研究[J].重庆医科大学学报,2010,35(05):695-699.

DUAN Dong, et al. Application of ^99mTc-EGFR-McAb to radioimmunoimaging for nude mice bearing human lung adenocarcinoma. [J]. Journal of Chongqing Medical University 35(5):695-699(2010)
[1]段东,朱玉泉,李少林.~(99m)Tc-EGFR-McAb用于荷人肺腺癌裸鼠的放射免疫显像研究[J].重庆医科大学学报,2010,35(05):695-699. DOI： 10.13406/j.cnki.cyxb.2010.05.039.

DUAN Dong, et al. Application of ^99mTc-EGFR-McAb to radioimmunoimaging for nude mice bearing human lung adenocarcinoma. [J]. Journal of Chongqing Medical University 35(5):695-699(2010) DOI： 10.13406/j.cnki.cyxb.2010.05.039.

摘要

目的:评价99mTc标记的表皮生长因子受体（Epidermal growth factor receptor

EGFR）单克隆抗体（Monoclone antibody

McAb）用于荷人肺腺癌裸鼠的放射免疫显像（Radioimmunoimaging

RII）效果

并进行相关比较分析。方法:①建立荷人肺腺癌裸鼠动物模型;②采用99mTc直接标记法标记抗EGFR的单克隆抗体

并对标记抗体的特性进行鉴定;③利用99mTc-EGFR-McAb进行荷人肺腺癌裸鼠模型的RII及体内分布研究。结果:99mTc对EGFR-McAb的标记率为91.5%±3.8%

标记抗体比活度为（2.8±0.2）MBq/μg

放射化学纯度为96.5%±3.2%。放射免疫显像结果显示

99mTc-EGFR-McAb对肿瘤组织有较高的亲和性

通过感兴趣区（Regional interest

ROI）技术测得T/NT比值为2.74±0.5

明显高于对照组（P<0.05）。标记抗体的体内分布结果与显像结果基本一致。结论:99mTc-EGFR-McAb用于荷人肺腺癌裸鼠的放射免疫显像能得到较理想的靶/非靶比值

EGFR是肺腺癌放射免疫显像研究值得推荐的目标抗原。

Abstract

Objective:To evaluate of the application of 99m Tc-EGFR-McAb to radioimmunoimaging for nude mice bearing lung adenocarcinoma and do correlation analysis.Methods:① The model of nude mice bearing lung adenocarcinoma was established;②The direct labeling method of 99mTc was applied to labele monoclonal antibodies for EGFR

and then the properties of the labeling antibodies were identified;③Radioimmunoimage and radioactivity distribution with 99mTc-EGFR-McAb on the animal model of nude mice bearing lung adenocarcinoma were studied.Results:The labeling rates of 99mTc for EGFR-McAb was 91.5% ±3.8%.Specific activity of the labeled antibody was(2.8±0.2)MBq/μg

and the radiochemical purity was 96.5%±3.2%.The tumor had high intake of the labeled antibodies according to radioimmunoimaging result.The T/NT relative value measured through ROI technique was 2.74±0.5

which was significantly higher than that of the control group(P<0.05).The body distribution results of the labeled antibodies and their imaging results were basically identical.Conclusion:The ideal target and non-target ratios could be obtained by applying 99mTc-EGFR-McAb to radioimmunoimaging of nude mice bearing lung adenocarcinoma

and EGFR may be an ideal target antigen to lung adenocarcinoma in the radioimmunoimaging study.

关键词

表皮生长因子受体放射免疫显像单克隆抗体肺肿瘤

Keywords

EGFRRadio immunoimagingmonoclonal antibodyLung cancer

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Application of 99mTc-EGFR-McAb to radioimmunoimaging for nude mice bearing human lung adenocarcinoma

DOI：10.13406/j.cnki.cyxb.2010.05.039

摘要

Abstract

关键词

Keywords

references

Application of ^99mTc-EGFR-McAb to radioimmunoimaging for nude mice bearing human lung adenocarcinoma