Determination of polyunsaturated fatty acid in human breast milk by reverse phase high performance liquid chromatography

FAGN Zhi-e; et al

doi:10.13406/j.cnki.cyxb.2010.05.005

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Determination of polyunsaturated fatty acid in human breast milk by reverse phase high performance liquid chromatography

更新时间：2024-01-17

- Determination of polyunsaturated fatty acid in human breast milk by reverse phase high performance liquid chromatography
- Journal of Chongqing Medical University Vol. 35, Issue 5, Pages: 699-702(2010)
- 作者机构：
  
  重庆医科大学药学院药物分析教研室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.05.005
  CLC： R151
- Published：2010，
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[1]方志娥,陆巍,曲斐,颜波,易珍奎,尚京川.母乳中多不饱和脂肪酸的RP-HPLC测定方法研究[J].重庆医科大学学报,2010,35(05):699-702.

FAGN Zhi-e, et al. Determination of polyunsaturated fatty acid in human breast milk by reverse phase high performance liquid chromatography. [J]. Journal of Chongqing Medical University 35(5):699-702(2010)
[1]方志娥,陆巍,曲斐,颜波,易珍奎,尚京川.母乳中多不饱和脂肪酸的RP-HPLC测定方法研究[J].重庆医科大学学报,2010,35(05):699-702. DOI： 10.13406/j.cnki.cyxb.2010.05.005.

FAGN Zhi-e, et al. Determination of polyunsaturated fatty acid in human breast milk by reverse phase high performance liquid chromatography. [J]. Journal of Chongqing Medical University 35(5):699-702(2010) DOI： 10.13406/j.cnki.cyxb.2010.05.005.

摘要

目的:建立测定母乳中亚油酸（Linoleic acid

LA）、α-亚麻酸（α-Linolenic acid

ALA）、花生四烯酸（Arachidonicacid

AA）、二十二碳六烯酸（Docosahexaenoic acid

DHA）的高效液相色谱法。方法:经液-液萃取并衍生提取母乳中的不饱和脂肪酸后

采用Agilent SB-C18（4.6mm×150mm

5μm）

流动相为乙腈:水=83:17

流速1.0ml/min

柱温30℃

紫外检测波长254nm进行。结果:ALA在50～350μg/ml、LA在200～1500μg/ml、DHA在10～80μg/ml和AA在20～160μg/ml范围内浓度与峰面积呈良好的线性关系

r均大于0.999;前三者的最低检测限均为0.2μg/ml

后者为0.4μg/ml;高、中、低浓度的平均回收率在92.65%～100.70%以内

精密度RSD≤5.67%。结论:本方法快速、准确且灵敏

满足临床和不同生物样品中多不饱和脂肪酸的含量测定。

Abstract

Objective:To establish a simultaneous determination of linoleic acid(LA)

α-linolenic acid(ALA)

arachidonic acid(AA)and docosahexaenoic acid(DHA)in human breast milk by reverse phase high performance liquid chromatography(RP-HPLC).Methods:The method of liquid-liquid extraction and derivation from human breast milk was used in a HPLC analysis.The column was Agilent SB-C18(4.6 mm×150 mm

5 μm).The mobile phase was acetonitrile:water(83:17

v/v)at 1.0 ml/min with UV detection at 254 nm and the column temperature was 30℃.Results:The standard curves were linear over the ranges of 50 ～350 μg/ml for α-linolenicacid

200～1500μg/mlforlinoleicacid

10～80 μg/ml for docosahexaenoic acid and 20 ～160 μg/ml for arachidonic acid

respectively.The good linearity can be obtained over their concentration range(r>0.999).The minimumdetectable limits of ALA

LA and DHA were all 0.2 μg/ml

and AA was 0.4 μg/ml.The average recovery of the method was 92.65%～100.70% and the RSD was lower than 5.67%.Conclusion:The HPLC method in this assay is rapid

direct and sensitive

which can be successfully applied to quantity the detection of polyunsaturated fatty acid of different biological samples as well as in clinic.

关键词

高效液相色谱法多不饱和脂肪酸母乳

Keywords

HPLCUnsaturated fatty acidbreast milk

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