Selection of formulation for IL-2 targeting liposome and determination of drug contents

WU Mei-chun; et al

doi:10.13406/j.cnki.cyxb.2010.04.046

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Selection of formulation for IL-2 targeting liposome and determination of drug contents

更新时间：2024-01-17

- Selection of formulation for IL-2 targeting liposome and determination of drug contents
- Journal of Chongqing Medical University Vol. 35, Issue 4, Pages: 591-593(2010)
- 作者机构：
  
  重庆医科大学药学院药物化学教研室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.04.046
  CLC： R927.2
- Published：2010，
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[1]吴镁春,朱莉,王驰.IL-2靶向脂质体的处方筛选及药物含量的测定[J].重庆医科大学学报,2010,35(04):591-593.

WU Mei-chun, et al. Selection of formulation for IL-2 targeting liposome and determination of drug contents. [J]. Journal of Chongqing Medical University 35(4):591-593(2010)
[1]吴镁春,朱莉,王驰.IL-2靶向脂质体的处方筛选及药物含量的测定[J].重庆医科大学学报,2010,35(04):591-593. DOI： 10.13406/j.cnki.cyxb.2010.04.046.

WU Mei-chun, et al. Selection of formulation for IL-2 targeting liposome and determination of drug contents. [J]. Journal of Chongqing Medical University 35(4):591-593(2010) DOI： 10.13406/j.cnki.cyxb.2010.04.046.

摘要

目的:筛选IL-2靶向脂质体的最佳处方

并对5-氟尿苷（5-fluorouridine

5-FUR）前药进行含量测定。方法:应用正交实验设计选择最佳处方配比;采用紫外分光光度法测定5-FUR前药的含量

检测波长为267nm。结果:筛选最佳处方:药脂比为1:10、磷脂与胆固醇的比例为3:1。5-FUP前药对照品浓度在0.002

.02mg/ml的范围内与吸收度线性关系良好

回归方程为y=16.357x+0.007

r=0.9997（n=3）

平均加样回收率为97.09%

RSD=2.05%（n=3）。样品中5-FUR前药含量为18.84

8.91μg/ml。结论:该处方设计合理

紫外分光光度法操作简便、稳定性好、结果可靠

可作为IL-2靶向脂质体中5-FUR前药含量测定方法。

Abstract

Objective:Selection ofthe optimal formulation for IL-2 targeting liposome and determination ofcontent for 5-FUR.Methods:orthogonal test method was used to selection the optimal formulation

UV spectrophotometry was used to determine the 5-FUR prodrug content

and the detection wavelength was at 267 nm.Results:The optimal formulation showed Drug/EPC 1∶10

EPC/Ch 3∶1.The absorbency and concentration of 5-FUR prodrug had good linearity in the range of 2.0～20.0μg/mL.The regression equation was y=16.357x+0.007

r= 0.999 7(n=3)

The 5-FUR prodrug content was 18.84～18.91μg/ml.The average recovery rate was 97.09%

and RSD=2.05%(n=3).Conclusion:Formulation was designed reasonablely.The method is simple

stable and reliable

which can be used to determine the 5-FUR prodrug in IL-2 targeting liposome.

关键词

5-FUR前药正交设计紫外分光光度法含量测定

Keywords

5-FUR prodrugorthogonal designUVspectrophotometrycontent determination

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