The separation and determination of ketoprofen enantiomers in plasma by chiral column

LI Xiao-mei; et al

doi:10.13406/j.cnki.cyxb.2010.04.045

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The separation and determination of ketoprofen enantiomers in plasma by chiral column

更新时间：2024-01-17

- The separation and determination of ketoprofen enantiomers in plasma by chiral column
- Journal of Chongqing Medical University Vol. 35, Issue 4, Pages: 586-590(2010)
- 作者机构：
  
  重庆医科大学药学院药物分析教研室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.04.045
  CLC： R917
- Published：2010，
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[1]李小梅,周凤,赵华,靳红卫.酮洛芬对映体的分离、分析方法的研究及其应用[J].重庆医科大学学报,2010,35(04):586-590.

LI Xiao-mei, et al. The separation and determination of ketoprofen enantiomers in plasma by chiral column. [J]. Journal of Chongqing Medical University 35(4):586-590(2010)
[1]李小梅,周凤,赵华,靳红卫.酮洛芬对映体的分离、分析方法的研究及其应用[J].重庆医科大学学报,2010,35(04):586-590. DOI： 10.13406/j.cnki.cyxb.2010.04.045.

LI Xiao-mei, et al. The separation and determination of ketoprofen enantiomers in plasma by chiral column. [J]. Journal of Chongqing Medical University 35(4):586-590(2010) DOI： 10.13406/j.cnki.cyxb.2010.04.045.

摘要

目的:考察酮洛芬（Ketoprofen

KT）对映体在多糖衍生物手性固定相的色谱行为

建立酮洛芬对映体的分离分析方法

并用于血浆样品中酮洛芬对映异构体的测定。方法:色谱柱Chiralpak AD（250mm×4.6mm

10μm

Daicel Chemicals）

流动相组成为正己烷:异丙醇:TFA（80:20:0.02）

检测波长254nm

流速1.0ml/min

柱温为25℃。结果:酮洛芬对映体在10min内实现分离

分离度大于2.0

对映体R和S分别在血药浓度0.1

0μg/ml范围内线性关系良好R-（-）（r=0.9998）

S-（+）（r=0.9996）

单一对映体的最低检测限为2ng（S/N=3）。结论:建立了用ChiralpakAD柱分离分析酮洛芬对映异构体的方法

可用于单一对映异构体的光学纯度检测及药理学研究。

Abstract

Objective:The separation effect for the enantiomers of ketoprofen on four diferent chiral columns was investigated and established a sensitive and stereospecific HPLC separation and determination method for ketoprofen enantiomers in animal plasma.Methods:The enantiomers of ketoprofen was resolved in the normal phase mode by using n-hexane∶isopropanol∶TFA(80∶20∶0.02)as mobile phase with UV detector at a wavelength of 254 nm.The flow rate was 1.0 ml/min.Results:Linear calibration curves were obtained in the range 0.1～10μg/ml for R-(+)-ketoprofen(r=0.999 8)and S-(+)-ketoprofen(r=0.999 6)in plasma.The detection limit(S/N=3) was 2 ng for each enantiomer.Conclusion:This method is simple

fast and can be used to detect optical purity of S-(+)-ketoprofen and monitor pharmacokinetic studies of ketoprofen enantiomers.

关键词

高效液相色谱法酮洛芬对映体手性分离

Keywords

HPLCKetoprofenEnantiomersChiral separation

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