Effect of gene Kai1 on the proliferation and cell cycle of laryngeal carcinoma Hep-2 cell line

WANG Hong-peng; et al

doi:10.13406/j.cnki.cyxb.2010.04.040

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Effect of gene Kai1 on the proliferation and cell cycle of laryngeal carcinoma Hep-2 cell line

更新时间：2024-01-17

- Effect of gene Kai1 on the proliferation and cell cycle of laryngeal carcinoma Hep-2 cell line
- Journal of Chongqing Medical University Vol. 35, Issue 4, Pages: 519-521(2010)
- 作者机构：
  
  1. 重庆医科大学附属第一医院耳鼻喉科
  2. 重庆医科大学附属第一医院胸心外科
  3. 重庆医科大学药学院药物化学教研室
  4. 重庆医科大学动物实验中心
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.04.040
  CLC： R739.65
- Published：2010，
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[1]王洪鹏,叶琳,黄闯,王驰,谭毅,陈鸿雁.Kai1对人喉癌Hep-2细胞株增殖及生长周期的影响[J].重庆医科大学学报,2010,35(04):519-521.

WANG Hong-peng, et al. Effect of gene Kai1 on the proliferation and cell cycle of laryngeal carcinoma Hep-2 cell line. [J]. Journal of Chongqing Medical University 35(4):519-521(2010)
[1]王洪鹏,叶琳,黄闯,王驰,谭毅,陈鸿雁.Kai1对人喉癌Hep-2细胞株增殖及生长周期的影响[J].重庆医科大学学报,2010,35(04):519-521. DOI： 10.13406/j.cnki.cyxb.2010.04.040.

WANG Hong-peng, et al. Effect of gene Kai1 on the proliferation and cell cycle of laryngeal carcinoma Hep-2 cell line. [J]. Journal of Chongqing Medical University 35(4):519-521(2010) DOI： 10.13406/j.cnki.cyxb.2010.04.040.

摘要

目的:探讨肿瘤转移抑制基因Kai1对人喉癌Hep-2细胞株增殖能力及生长周期的影响。方法:本研究以喉癌Hep-2细胞株为研究对象

将携带有Kai1基因的重组腺病毒（rAd-Kai1）进行扩增、纯化并测定其滴度

用适量rAd-Kai1转染人喉癌Hep-2细胞株

利用RT-PCR技术对转染效果进行检测

以未转染Kai1的喉癌细胞株为对照组

用四甲基偶氮唑蓝（MTT）法、流式细胞术测定转染组细胞株的增殖抑制率和细胞周期。结果:rAd-Kai1经扩增、纯化后

滴度可达1010PFU/ml

当病毒量为30MOI时

对Hep-2细胞的转染率达93%以上。MTT实验显示转染Kai1的Hep-2细胞株体外增殖能力明显弱于对照组（P<0.01）

抑制率可达29%。流式细胞术显示与对照组比较

转染组G0/G1期细胞数量增加

S期细胞数量减少

差异有显著性（P<0.05）。结论:通过重组腺病毒介导

成功地将Kai1基因转入喉癌Hep-2细胞株

Kai1对喉癌细胞体外增殖能力具有抑制作用

其机制可能是将Hep-2细胞生长周期阻滞于G0/G1期

提示:Kai1抑癌基因可能通过阻滞肿瘤细胞生长周期实现抑制肿瘤细胞增殖。

Abstract

Objective:To study the effect of gene Kai1 on the proliferation and cell cycle of laryngeal carcinoma Hep-2 cell line.Methods:The Hep-2 cell line was used as the subject

the expansion purification and the concentration of the Kai1 gene was tested

and the gene was transferred into Hep-2 cell line by adenovirus.The non-transferred group was used as a control.By means of MTT the effect of Kai1 gene on the cell proliferation of Hep-2 cell line was tested and by means of RT-PCR the expression of Kai1 in transfected cell was tested;cell cycle of Hep-2 cells was assayed by flow cytometry.Results:The concentration of Kai1 gene can be 1010 PFU/ml after expansion and purification

when the concentration of the adenovirus was 30 MOI

the transfection rate of Hep-2 cell line can be above 93%.MTT shows the cell proliferation was slower and less of the Kai1-Hep-2 cell line compared to the control group(P<0.01).The inhibition rate can up to 29%.Flow cytometry showed the number of Kai1-Hep-2 cells in G0/G1 phase was elevated

and the number in S phase was decreased.Conclusion:The Kai1 gene had the effect of inhibiting the cell proliferation of Hep-2 cell line and the possible machanism would be the cycle pattern was changed after Hep-2 cells was transfected with Kai1 gene.

关键词

KAI1/CD82Hep-2喉癌腺病毒转染增殖生长周期

Keywords

KAI1/CD82Hep-2laryngeal carcinomaadenovirustransfectionproliferationCell cycle

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