Objective:To investigate the effect of allitride injection preconditioning and ischemic preconditioning on renal ischemia/ reperfusion(I/R)injury in rats.Methods:Rat’s I/R injury model was established.A total of 75 rats were randomized into five groups(n=15

in each);control group(CON)

I/R control group(IR)

ischemic preconditioninggroup(IPC)

allitride preconditioninggroup(APC)

allitride preconditioning and ischemic preconditioning group(AIPC).Kidney tissue and blood specimen were collected from all groups 24 hours after operation.The changes of the renal pathological morphology were observed by hematoxylin-eosine(HE)staining.The levels of serum creatinine(Cr)and urea nitrogen(BUN) were determined.The levels of malonaldehyde(MDA)were determined by thibabituric acid(TBA).The levels of superoxide dismutase(SOD)were determined by xanthine oxidase.Intercellular adhesion molecule-1(ICAM-1)expression in kidney was tested by immunohistoehemistry.Results:IPC or APC group had less necrosis in HE staining than IR group.BUN

Cr

MDA contents in IPC or APC group are less than that in IR group(P<0.01).SOD activity in IPC or APC group is higher than IR group(P<0.01).ICAM-1 expression in kidney in IPC or APC group is lower than IR group(P<0.01).After AIPC process

all indicators substantially differed from those only after IPC or APC(P<0.01).Conclusion:Ischemic preconditioning or allitride preconditioning would protect kidney IR injury

and that the two factor would have synergy in protecting kidney IR injury

probably by down regulating ICAM-1 expression.