Amperometie immunoassay biosensor for urinary trypsinogen-2

ZHENG Jun; et al

doi:10.13406/j.cnki.cyxb.2010.03.050

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Amperometie immunoassay biosensor for urinary trypsinogen-2

更新时间：2024-01-17

- Amperometie immunoassay biosensor for urinary trypsinogen-2
- Journal of Chongqing Medical University Vol. 35, Issue 3, Pages: 382-385(2010)
- 作者机构：
  
  1. 重庆医科大学检验系、临床检验诊断学教育部重点实验室
  2. 四川省医学科学院附属医院检验科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.03.050
  CLC： TP212.3
- Published：2010，
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[1]郑军,马霄,刘成桂.尿胰蛋白酶原-2电流型免疫生物传感器的研究[J].重庆医科大学学报,2010,35(03):382-385.

ZHENG Jun, et al. Amperometie immunoassay biosensor for urinary trypsinogen-2. [J]. Journal of Chongqing Medical University 35(3):382-385(2010)
[1]郑军,马霄,刘成桂.尿胰蛋白酶原-2电流型免疫生物传感器的研究[J].重庆医科大学学报,2010,35(03):382-385. DOI： 10.13406/j.cnki.cyxb.2010.03.050.

ZHENG Jun, et al. Amperometie immunoassay biosensor for urinary trypsinogen-2. [J]. Journal of Chongqing Medical University 35(3):382-385(2010) DOI： 10.13406/j.cnki.cyxb.2010.03.050.

摘要

目的:研制一种检测尿胰蛋白酶原-2（Urinary Trypsinogen-2

TSG-Ⅱ）的新型电流型免疫生物传感器。方法:将Nafion吸附到玻碳电极表面

并通过静电吸附和共价键合作用将硫堇和纳米金进行层层自组装

然后通过形成的纳米金单层吸附TSG-Ⅱ抗体

最后用辣根过氧化物酶封闭电极上的非特异吸附位点。结果:该免疫生物传感器对TSG-Ⅱ的响应特性良好

其线性检测范围为6.0～100μg/L;检出限为3.5μg/L。结论:该电流型免疫生物传感器具有灵敏度高、特异性好等优点

为建立一种检测TSG-Ⅱ新方法奠定了基础。

Abstract

Objective:To develop a novel amperometic immunosensor for the determination of Urinary Trypsinogen-2(TSG-Ⅱ).Methods:the immunosensor was developed by modifying Nafion film on the glassy carbon electrode(GCE)

then thionine and colloidal gold were immobilized by static electricity and covalent bonding self-assembly.And then the antibody of Urinary TSG-Ⅱwas absorbed on the surface of nano-gold monolayer.Finally

Horseradish peroxidase(HRP) blocked the non-specific adsorption site on the electrode.Result:Experiment results showed that the good linear relations between peak current and concentration of TSG-Ⅱ were established in the range of 6.0-100μg/L.The detection limit was 3.5μg/L.Conclusion:The amperometie immunoassay biosensor has high sensitivity and good specificity and it has laid the foundation to establish a new method for detection of TSG-Ⅱ.

关键词

尿胰蛋白酶原-2免疫生物传感器纳米金硫堇壳聚糖

Keywords

TSG-ⅡImmunosensorColloidal goldThioninechitosan

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