Analysis and identification of human epidermal proteins associated with photoaging

doi:10.13406/j.cnki.cyxb.2010.03.045

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Analysis and identification of human epidermal proteins associated with photoaging

更新时间：2024-01-17

- Analysis and identification of human epidermal proteins associated with photoaging
- Journal of Chongqing Medical University Vol. 35, Issue 3, Pages: 385-388(2010)
- 作者机构：
  
  重庆医科大学附属第一医院皮肤科重庆医科大学分子医学与肿瘤研究中心
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.03.045
  CLC： R758.1
- Published：2010，
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[1]李文静,李惠,黄欣,陈爱军,龙亭.人表皮光老化相关蛋白的蛋白质组分析与鉴定[J].重庆医科大学学报,2010,35(03):385-388.

Analysis and identification of human epidermal proteins associated with photoaging. [J]. Journal of Chongqing Medical University 35(3):385-388(2010)
[1]李文静,李惠,黄欣,陈爱军,龙亭.人表皮光老化相关蛋白的蛋白质组分析与鉴定[J].重庆医科大学学报,2010,35(03):385-388. DOI： 10.13406/j.cnki.cyxb.2010.03.045.

Analysis and identification of human epidermal proteins associated with photoaging. [J]. Journal of Chongqing Medical University 35(3):385-388(2010) DOI： 10.13406/j.cnki.cyxb.2010.03.045.

摘要

目的:以老年人正常皮肤为研究对象

分析曝光部位及非曝光部位的表皮差异蛋白

筛选出表皮细胞中与皮肤光化相关的蛋白。方法:从分离所得的不同部位老年人表皮中提取蛋白质

并进行2-DE分离

PDQUEST软件分析凝胶上差异蛋白点

进一步采用MALDI-TOF质谱分析及数据库查询

鉴定差异表达蛋白;并在蛋白质水平进行Western-blotting验证差异候选蛋白。结果:通过分析比较两组表皮双向电泳图谱

确定了15个差异蛋白点

经MALDI-TOF分析及数据库查询

成功鉴定了其中5个差异表达蛋白

发现热休克蛋白B1（Heat shock protein B1

HSPB1）、膜联蛋白A2（Annexin A2

ANXA2）、角蛋白80（Keratin

typeⅡcytoskeletal80

K2C80）、丝裂原激活的蛋白激酶3（Mitogen-activated protein kinase3

MK03）在老年人曝光部位的表皮中高表达

而谷胱甘肽S转移酶A2（Glutathione S-transferase A2

GSTA2）在非曝光部位表皮中高表达。结论:老年人正常皮肤曝光部位和非曝光部位的表皮存在差异蛋白

经分析和鉴定的5种蛋白质与皮肤的光老化有密切的关系

为进一步研究表皮细胞在光老化过程中的作用及分子机制奠定基础。

Abstract

Objective:This study is to analyze the differential proteins expressed in the normal elderly exposed epidermis and the non-exposed epidermis.Methods:The proteins were extracted from the exposed and non-exposed elderly separated epidermis

and were submitted to two-dimensional gel electrophoresis.Then their electrophoretic patterns were analyzed by the PDQUEST software and identified by MALDI-TOF MS.The following steps were database query and probation using Western-blotting.Results:By comparison the two groups of two-dimensional electrophoresis patterns

15 differential protein spots were determined

in which 5 differential protein spots were successfully identified by MALDI-TOF analysis and database queries

and 4 proteins

Heat shock protein B1(HSPB1)

Annexin A2(ANXA2)

Keratin

typeⅡ cytoskeletal 80(K2C80)

Mitogen-activated protein kinase 3(MK03)

were highly expressed in exposed group while Glutathione S-transferase A2(GSTA2) was highly expressed in non-exposed group.Conclusion:The changes in expression of 5 identified proteins may have a close relationship with photoaging

that helps revealing molecular mechanism of epidermal cells in photoaging.

关键词

光老化表皮蛋白质组

Keywords

PhotoagingEpidermisProteome

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