Effects and mechanism of triptolide on retinal gonlion cells of rats with EAE

TAN Wei; et al

doi:10.13406/j.cnki.cyxb.2010.03.042

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Effects and mechanism of triptolide on retinal gonlion cells of rats with EAE

更新时间：2024-01-17

- Effects and mechanism of triptolide on retinal gonlion cells of rats with EAE
- Journal of Chongqing Medical University Vol. 35, Issue 3, Pages: 364-367(2010)
- 作者机构：
  
  重庆医科大学附属第一医院眼科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.03.042
  CLC： R774.6
- Published：2010，
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[1]谭蔚,李平华.雷公藤甲素对EAE大鼠视网膜神经节细胞的作用及机制[J].重庆医科大学学报,2010,35(03):364-367.

TAN Wei, et al. Effects and mechanism of triptolide on retinal gonlion cells of rats with EAE. [J]. Journal of Chongqing Medical University 35(3):364-367(2010)
[1]谭蔚,李平华.雷公藤甲素对EAE大鼠视网膜神经节细胞的作用及机制[J].重庆医科大学学报,2010,35(03):364-367. DOI： 10.13406/j.cnki.cyxb.2010.03.042.

TAN Wei, et al. Effects and mechanism of triptolide on retinal gonlion cells of rats with EAE. [J]. Journal of Chongqing Medical University 35(3):364-367(2010) DOI： 10.13406/j.cnki.cyxb.2010.03.042.

摘要

目的:观察雷公藤甲素（Triptolide

TP）对实验性自身免疫性脑脊髓炎（Experimental autoimmune encephalomyelitis

EAE）大鼠视网膜神经节细胞（Retinal ganglion cells

RGCs）的作用及可能机制。方法:45只雌性SD大鼠随机分成3组:正常组、EAE组、TP组。豚鼠脊髓全匀浆加完全福氏佐剂免疫SD大鼠建立EAE模型

TP组于免疫当天至第14d雷公藤甲素0.1mg/kg每日腹腔注射

分别于EAE大鼠发病后第1d（初期）、7d（高峰期）、14d及30d（恢复期）

观察各个时间段大鼠视神经的病理变化

免疫组化法检测视网膜单核细胞趋化因子-1（Monocyte chemokine protein-1

MCP-1）、胶质纤维酸性蛋白（Glial fibrillaryacidic protein

GFAP）的表达

TUNEL法检测RGCs凋亡。结果:EAE大鼠在发病后第7d左右达到高峰

MCP-1发病初期出现在EAE大鼠视网膜上

发病后第7d开始明显下降

病理改变视神经可见炎性细胞侵润及脱髓鞘

TP组大鼠MCP-1及GFAP表达较EAE组降低（P<0.01）

神经节细胞凋亡数量与EAE组比较无明显差别（P>0.05）。结论:雷公藤甲素能减轻视神经的炎症反应

下调视网膜MCP-1及GFAP的表达

但不能直接减少视网膜神经节细胞凋亡。

Abstract

Objective:To observe the effects of triptolide(TP) on retinal gonlion cells of SD rats with experimental autoimmune encephalomyelitis(EAE)

and to analyze its possible mechanism.Methods:EAE models were induced in SD rats

the TP group received triptolidedayly intraperitoneal injection from immunization day to 14 days.The pathological changes in optic nerve were observed

the expression of MCP-1

GFAP and the apoptosis of RGCs in retina were detected.Results:Demyelination and inflammatory cell infiltration showed in optic nerve

MCP-1 appearred in the retina at the initial stage

and begine to decline after onset 7 days.The expression of MCP-1 and GFAP were lower in TP group

compared with the EAE group with statistically significant difference(P<0.01)

while with no significant difference on the apoptosis of RGCs(P>0.05).Conclusion:Triptolide inhibit the expression of MCP-1 and GFAP

have no significant effects on the apoptosis of RGCs.

关键词

实验性视神经炎单核细胞趋化因子-1视网膜神经节细胞雷公藤甲素

Keywords

Experimental optic neuritisMonocyte chemokine protein-1Retinal gonlion cellsTriptolide

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Related Author

LI Yan

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Related Institution

Department of Natural Medicinal Chemistry,College of Pharmacy,Chongqing Medical University

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