Objective:To investigate the effects of tumorigenicity and vascular generation of nude mice on antisence vascular endothelial growth factor-C(antisense VEGF-C) gene transinfection of human gastric carcinoma cell by observing the vegetation process of implanted gastric tumor.Methods:30 nude mice were divided into three groups randomly:transgenic anti-sense VEGF-C group

transgenic pure liposome group and non-transgenic group.The cell suspension of gastric carcinoma cells SGC-7901 non-transinfected or transinfected by anti-sense VEGF-C gene or pure liposome were subcutaneously injected into those mice.After injection

the size and growth velocity of those neoplasma were detected.The Microlymphatic vessel density(MLVD) and microvessel density(MVD) of tumor were detected too.Results:The neoplasma grew more slowly and was smaller in the mice in those transinfected by anti-sense VEGF-C gene than in non-transgenic ones(P<0.05).At the end of the 1st

2nd and 3rd week

there was significant difference between the anti-sense VEGF-C group and the other groups on lymphangiogenesis of the tumor(P<0.05).the MLVD in the anti-sense VEGF-C group was 4±2.2/HF

6±3.1/HF and 9±2.7/HF respectively.In the transgenic pure liposome group

it was 6±8.7/HF

9±3.5/HF and 18±7.2/HF respectively.In the non-transgenic group

it was 7.0±4.9/HF

9.0±6.4/HF and 19.0±6.5/HF respectively.But it had little difference on angiogenesis of the tumor in the 3 groups(P>0.05).Conclusions:The antisense VEGF-C gene suppress the tumorigenicity and lymphangiogenesis in the human SGC-7901 gastric carcinoma cell apparently.but it had little effects on angiogenesis of the tumor.VEGF-C might participate the lymphangiogenesis of the gastric tumor.