The effects of siRNA-mediated Plk1 depletion on COS-7 cells

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The effects of siRNA-mediated Plk1 depletion on COS-7 cells

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- The effects of siRNA-mediated Plk1 depletion on COS-7 cells
- Journal of Chongqing Medical University Vol. 35, Issue 3, Pages: 324-327(2010)
- 作者机构：
  
  1. 重庆医科大学附属第一医院耳鼻咽喉科
  2. 重庆医科大学分子医学与肿瘤研究中心
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.03.028
  CLC： R73-3
- Published：2010，
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[1]朱江,洪苏玲,柯霞,兰欢,卜友泉.siRNA介导的Plk1表达沉默对COS-7细胞影响的研究[J].重庆医科大学学报,2010,35(03):324-327.

ZHU Jiang, et al. The effects of siRNA-mediated Plk1 depletion on COS-7 cells. [J]. Journal of Chongqing Medical University 35(3):324-327(2010)
[1]朱江,洪苏玲,柯霞,兰欢,卜友泉.siRNA介导的Plk1表达沉默对COS-7细胞影响的研究[J].重庆医科大学学报,2010,35(03):324-327. DOI： 10.13406/j.cnki.cyxb.2010.03.028.

ZHU Jiang, et al. The effects of siRNA-mediated Plk1 depletion on COS-7 cells. [J]. Journal of Chongqing Medical University 35(3):324-327(2010) DOI： 10.13406/j.cnki.cyxb.2010.03.028.

摘要

目的:研究（Polo-like kinase1

Plk1）表达被抑制后对COS-7细胞的影响

探讨Plk1作为分子靶点用于肿瘤治疗的潜在价值。方法:化学合成法合成特异性的短链Plk1siRNA

脂质体转染法将短链siRNA分别导入COS-7细胞;采用RT-PCR和Western blot法检测siRNA对Plk1表达的抑制效果;采用胎盘兰染色结合细胞计数法、FACS分析siRNA介导的Plk1表达抑制对COS-7癌细胞生长增殖、细胞周期的影响。结果:半定量RT-PCR和蛋白质印迹分析结果表明

合成的特异性短链Plk1siRNA在mRNA和蛋白质水平上均能高效、特异性地抑制COS-7细胞中Plk1的表达。细胞生长分析结果表明

siRNA介导的Plk1表达沉默导致COS-7细胞的生长增殖受到显著抑制。FACS分析结果表明

Plk1表达被抑制后

出现细胞周期停滞

大量细胞呈现sub-G1期时相。结论:siRNA介导的Plk1表达沉默显著抑制COS-7细胞生长增殖

是一个潜在的肿瘤治疗靶点。

Abstract

Objective:To investigate the effects of siRNA-mediated Plk1 depletion on COS-7 cells and the therapeutic potential of Plk1 in cancer treatment.Methods:Small interfering RNA(siRNA) were used to specifically deplete Plk1 in COS-7 cells.RT-PCR and immol/Lunoblotting analysis were used to verify the inhibitory effect of siRNA against Plk1.Trypan blue exclusion assay and FACS analysis were conducted to determine the effects of Plk1 depletion on the growth and cell cycle profile of COS-7 cells.Results:Synthetic siRNA duplexes against Plk1 were introduced into COS-7 cells

which subsequently resulted in a significant inhibition in Plk1 expression in the cells.Cell growth assay indicated that siRNA-mediated Plk1 depletion significantly inhibited the proliferation of COS-7 cells.FACS analysis revealed that Plk1 depletion caused cell cycle arrest

resulting into large population of sub-G1 cells.Conclusion:siRNA-mediated Plk1 depletion significantly inhibited the proliferation of COS-7 cells suggesting the therapeutic potential of Plk1 in cancer treatment.

关键词

Plk1siRNA细胞周期细胞生长

Keywords

Plk1siRNAcell cyclecell growth

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