Dynamic observation on Th1/Th2 type immune response in mice immunized with recombinant Bb-Eg95-EgA31 vaccine of Echinococcus granulosus

ZHOU Bi-ying; et al

doi:10.13406/j.cnki.cyxb.2010.03.005

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Dynamic observation on Th1/Th2 type immune response in mice immunized with recombinant Bb-Eg95-EgA31 vaccine of Echinococcus granulosus

更新时间：2024-01-17

- Dynamic observation on Th1/Th2 type immune response in mice immunized with recombinant Bb-Eg95-EgA31 vaccine of Echinococcus granulosus
- Journal of Chongqing Medical University Vol. 35, Issue 3, Pages: 327-331(2010)
- 作者机构：
  
  重庆医科大学附属第一医院传染病寄生虫病研究所
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.03.005
  CLC： R392
- Published：2010，
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[1]周必英,陈雅棠,李文桂,杨梅.细粒棘球绦虫重组Bb-Eg95-EgA31疫苗免疫小鼠后Th1/Th2型免疫应答的动态观察[J].重庆医科大学学报,2010,35(03):327-331.

ZHOU Bi-ying, et al. Dynamic observation on Th1/Th2 type immune response in mice immunized with recombinant Bb-Eg95-EgA31 vaccine of Echinococcus granulosus. [J]. Journal of Chongqing Medical University 35(3):327-331(2010)
[1]周必英,陈雅棠,李文桂,杨梅.细粒棘球绦虫重组Bb-Eg95-EgA31疫苗免疫小鼠后Th1/Th2型免疫应答的动态观察[J].重庆医科大学学报,2010,35(03):327-331. DOI： 10.13406/j.cnki.cyxb.2010.03.005.

ZHOU Bi-ying, et al. Dynamic observation on Th1/Th2 type immune response in mice immunized with recombinant Bb-Eg95-EgA31 vaccine of Echinococcus granulosus. [J]. Journal of Chongqing Medical University 35(3):327-331(2010) DOI： 10.13406/j.cnki.cyxb.2010.03.005.

摘要

目的:动态观察细粒棘球绦虫（Echinococcus granulosus

Eg）重组双歧杆菌（Bb）-Eg95-EgA31疫苗免疫小鼠后Th1/Th2型免疫应答的变化。方法:将5×108克隆形成单位（CFU）和5×105CFU疫苗分别采用口服灌胃和鼻腔内接种免疫BALB/c鼠

在免疫后2、4、6、8、10、12、14、16、18和20周各剖杀4只小鼠

收集血清

用ELISA法测定血清IgG及其亚类和IgE水平;无菌取脾

用ELISA法检测脾淋巴细胞培养上清液中IFN-γ、IL-12、TNF-α和IL-10水平。结果:口服免疫组小鼠血清IgG、IgG2a、IgG2b、IgG1、IgG3和IgE水平分别在免疫后8～10周、2～20周、2～20周、4～8周、6～12周和10周显著升高

脾淋巴细胞培养上清液中IFN-γ、IL-12、TNF-α和IL-10水平分别在免疫后2～16周、2～12周、2～6周和4～12周显著升高;鼻腔内接种组小鼠血清IgG、IgG2a、IgG2b、IgG1、IgG3和IgE水平分别在免疫后4～10周、4～20周、2～20周、2～12周、4～12周和10～12周显著升高

脾淋巴细胞培养上清液中IFN-γ、IL-12、TNF-α和IL-10水平分别在免疫后2～8周、2～12周、2～8周和6～16周显著升高。结论:细粒棘球绦虫重组Bb-Eg95-EgA31疫苗可诱导小鼠产生1个Th1/Th2混合型免疫应答。口服免疫和鼻腔内接种是两种较好的免疫途径

且前者优于后者。

Abstract

Objective:To dynamically observe changes of Th1/Th2 type immune responses in mice immunized with recombinant Bifidobacteria bifidum(Bb)-Eg95-EgA31 vaccine of Echinococcus granulosus(Eg).Methods:BALB/c mice were vaccinated by 5×108 colony forming unit(CFU) orally and 5×105CFU intranasally respectively.Mice were killed on week 2

18 and 20 after immunization respectively

sera were collected to measure the levels of IgG

its subclasses and IgE by enzyme linked immunosorbent assay(ELISA);Spleens were removed to determine the levels of IFN-γ

IL-12

TNF-αand IL-10 of splenocyte culture supernatant by ELISA.Results:Oral immunization mice showed higher levels of IgG

IgG2a

IgG2b

IgG1

IgG3 and IgE on the 8～10th week

2～20th week

4～8th week

6～12th week and 10th week respectively

while IFN-γ

IL-12

TNF-αand IL-10 on the 2～16th week

2～12th week

2～6th week and 4～12th week post vaccination respectively;Intranasal immunization mice showed higher levels of IgG

IgG2a

IgG2b

IgG1

IgG3 and IgE during the 4～10th week

4～20th week

2～20th week

2～12th week

4～12th week and 10～12th week respectively

while IFN-γ

IL-12

TNF-αand IL-10 on the 2～8th week

2～12th week

2～8th week and 6～16th week post vaccination respectively.Conclusion:Mixed Th1/Th2 type immune responses may be induced in mice immunized with the recombinant Bb-Eg95-EgA31 vaccine of Echinococcus granulosus.Oral and intranasal inoculation are two better immune routes

and the former is better than the latter.

关键词

细粒棘球绦虫重组Bb-Eg95-EgA31疫苗免疫球蛋白Th1Th2细胞因子

Keywords

Echinococcus granulosusRecombinant Bb-Eg95-EgA31 vaccineImmunoglobulinsTh1Th2cytokines

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