Analysis of the expression of ornithine decarboxylase antizyme inhibitor in mouse tissues

WANG Zeng-chan; et al

doi:10.13406/j.cnki.cyxb.2010.03.002

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Analysis of the expression of ornithine decarboxylase antizyme inhibitor in mouse tissues

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- Analysis of the expression of ornithine decarboxylase antizyme inhibitor in mouse tissues
- Journal of Chongqing Medical University Vol. 35, Issue 3, Pages: 332-334(2010)
- 作者机构：
  
  重庆医科大学感染性疾病分子生物学重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.03.002
  CLC： Q55
- Published：2010，
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[1]王增产,张小花,万涛,王李英,刘湘,汤华.鸟氨酸脱羧酶抗酶抑制子小鼠体内的表达分析[J].重庆医科大学学报,2010,35(03):332-334.

WANG Zeng-chan, et al. Analysis of the expression of ornithine decarboxylase antizyme inhibitor in mouse tissues. [J]. Journal of Chongqing Medical University 35(3):332-334(2010)
[1]王增产,张小花,万涛,王李英,刘湘,汤华.鸟氨酸脱羧酶抗酶抑制子小鼠体内的表达分析[J].重庆医科大学学报,2010,35(03):332-334. DOI： 10.13406/j.cnki.cyxb.2010.03.002.

WANG Zeng-chan, et al. Analysis of the expression of ornithine decarboxylase antizyme inhibitor in mouse tissues. [J]. Journal of Chongqing Medical University 35(3):332-334(2010) DOI： 10.13406/j.cnki.cyxb.2010.03.002.

摘要

目的:分析鸟氨酸脱羧酶抗酶抑制子（Ornithine decarboxylase antizyme inhibitor

AZI）在小鼠体内的表达情况。方法:RT-PCR

Northern blot

免疫组化染色。结果:RT-PCR

Northern blot揭示AZT基因（Oazin）的表达是全身性的

即各器官都表达。特别是脑

心

肾脏

肌肉

胸腺

睾丸。免疫组化染色显示AZT蛋白在心瓣膜细胞

肾小管周围的间质细胞以及肝细胞的间质细胞中高表达。结论:用RT-PCR

Northern blot以及免疫组化染色法揭示了AZT在RNA和蛋白水平小鼠体内中的表达情况。

Abstract

Objective:Analysis of the expression of Ornithine decarboxylase antizyme inhibitor in mouse tissues.Methods:RT-PCR

Northern blot

immunostaining.Results:RT-PCR

Northern blot reveal that the expression of Ornithine decarboxylase antizyme inhibitor gene(AZI) is ubiquitous

especially high in brain

heart

kidney

muscle

thymus and testis.Immunostaining reveals that AZI expresses on valve cells in heart

mesenchyme cells in kidney and liver.Conclusion:The expression of AZI in mouse tissues was identified by using RT-PCR

Northern blot and immunostaining.

关键词

鸟氨酸脱羧酶抗酶抑制子表达

Keywords

Ornithine decarboxylase antizyme inhibitor(AZI)expression

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Related Institution

Department of Pediatric Research Institute,the Children’s Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders,Chongqing Key Laboratory of Pediatrics,Chongqing International Science and Technology Cooperation Center for Child Development and Disorders

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