Biological effect of ultrasound-mediated microbubble destruction on sertoli cells

ZHANG bo; et al

doi:10.13406/j.cnki.cyxb.2010.02.046

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Biological effect of ultrasound-mediated microbubble destruction on sertoli cells

更新时间：2024-01-17

- Biological effect of ultrasound-mediated microbubble destruction on sertoli cells
- Journal of Chongqing Medical University Vol. 35, Issue 2, Pages: 175-178(2010)
- 作者机构：
  
  1. 重庆医科大学附属第二医院泌尿外科
  2. 重庆医科大学超声影像学研究所
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.02.046
  CLC： R312
- Published：2010，
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[1]张波,张唯力,林艳君,王志刚.超声靶向微泡破坏对支持细胞的生物学效应研究[J].重庆医科大学学报,2010,35(02):175-178.

ZHANG bo, et al. Biological effect of ultrasound-mediated microbubble destruction on sertoli cells. [J]. Journal of Chongqing Medical University 35(2):175-178(2010)
[1]张波,张唯力,林艳君,王志刚.超声靶向微泡破坏对支持细胞的生物学效应研究[J].重庆医科大学学报,2010,35(02):175-178. DOI： 10.13406/j.cnki.cyxb.2010.02.046.

ZHANG bo, et al. Biological effect of ultrasound-mediated microbubble destruction on sertoli cells. [J]. Journal of Chongqing Medical University 35(2):175-178(2010) DOI： 10.13406/j.cnki.cyxb.2010.02.046.

摘要

目的:探讨超声靶向微泡破坏对体外培养大鼠支持细胞的影响。方法:分离与培养大鼠支持细胞

制作细胞悬液

添加0.01 ml微泡造影剂后即行超声辐照

超声辐照时间为60 s

输出声强为0.5 W/cm

频率为1 MHz

分别取照射后5 min

2、6、12、24 h作为分组时间点

在各时间点及进行波形蛋白免疫组化染色、测定细胞超氧化物歧化酶（Superoxide dismutase

SOD）、丙二醛（Malondialdehyde

MDA）以及细胞计数。结果:超声联合微泡可以降低支持细胞内波形蛋白的表达

引起细胞内早期MDA含量可逆性升高

SOD活性下降

后期（24 h）MDA含量恢复正常

SOD活性升高

而支持细胞的存活不受影响。结论:超声靶向微泡破坏支持细胞的结构和功能引起可逆性损伤

通过降低支持细胞紧密连接蛋白的表达从而开放血睾丸屏障

继而影响生精细胞功能

为男性避孕的研究提供新的思路。

Abstract

Objective:To investigate the effect of ultrasound-mediated microbubble destruction on the Sertoli cells of rat testis in vitro. Methods:Sertoli cells were isolated and cultured from SD rat testis.After ultrasound contrast agent was added into cell suspension

ultrasound was performed for 60 s at the frequency of 1 MHz

and the intensity of 0.5 W/cm2.At the point of 5 min

and 24 h after ultrasound performed

the expression of vimentin was detected by immunohistochemical staining

SOD

MDA in cell were detected and the cells were counted.Results:Ultrasound-mediated microbubble destruction decreased the expression of vimentin

increased MDA and decreased SOD at earlier time

but at later time(24 h)

MDA became normal

SOD increased and had no effect on Sertoli cells’survival. Conclusion:Ultrasound-mediated microbubble destruction can cause damage on structure and function of Sertoli cells reversibly

which can provide a new method for the study of male contraception.

关键词

超声微泡波形蛋SODMDA男性避孕

Keywords

Ultrasound-mediated microbubbleVimentinSODMDAMale contraception

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