Study of NS398 reversing the antergy of lysophosphatidic acid for the inhibiting effect of cisplatin in the proliferation of huanman ovarian cancer

XIONG Xue-hua; et al

doi:10.13406/j.cnki.cyxb.2010.02.035

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Study of NS398 reversing the antergy of lysophosphatidic acid for the inhibiting effect of cisplatin in the proliferation of huanman ovarian cancer

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- Study of NS398 reversing the antergy of lysophosphatidic acid for the inhibiting effect of cisplatin in the proliferation of huanman ovarian cancer
- Journal of Chongqing Medical University Vol. 35, Issue 2, Pages: 209-212(2010)
- 作者机构：
  
  重庆医科大学基础医学院病理学教研室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2010.02.035
  CLC： R737.31
- Published：2010，
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[1]熊学华,彭真年.NS398逆转溶血磷脂酸拮抗顺铂抑制卵巢癌细胞增殖作用的研究[J].重庆医科大学学报,2010,35(02):209-212.

XIONG Xue-hua, et al. Study of NS398 reversing the antergy of lysophosphatidic acid for the inhibiting effect of cisplatin in the proliferation of huanman ovarian cancer. [J]. Journal of Chongqing Medical University 35(2):209-212(2010)
[1]熊学华,彭真年.NS398逆转溶血磷脂酸拮抗顺铂抑制卵巢癌细胞增殖作用的研究[J].重庆医科大学学报,2010,35(02):209-212. DOI： 10.13406/j.cnki.cyxb.2010.02.035.

XIONG Xue-hua, et al. Study of NS398 reversing the antergy of lysophosphatidic acid for the inhibiting effect of cisplatin in the proliferation of huanman ovarian cancer. [J]. Journal of Chongqing Medical University 35(2):209-212(2010) DOI： 10.13406/j.cnki.cyxb.2010.02.035.

摘要

目的:观察环氧化酶-2（Cyclooxygenase-2

COX-2）抑制剂NS398对溶血磷脂酸（Lysophosphatidic acid

LPA）拮抗顺铂抑制卵巢癌细胞细胞增殖作用的影响

并探讨其可能机制。方法:体外培养人卵巢癌细胞3AO

MTT法检测NS398对LPA拮抗顺铂抑制3AO细胞增殖的影响;流式细胞仪检测细胞周期变化;RT-PCR检测LPA单独或合用NS398对3AO细胞表达COX-2的影响。结果:LPA对顺铂抑制卵巢癌细胞增殖有拮抗作用

并降低G

期的细胞比率;联合用NS398后

卵巢癌细胞的生长显著受抑制

同时G

期细胞比率上升;RT-PCR结果显示40μmol/L LPA能促进COX-2的表达

而合用NS398后COX-2表达降低。结论:NS398可能通过抑制COX-2表达

实现逆转LPA拮抗顺铂抑制卵巢癌细胞细胞增殖的作用。

Abstract

Objective:To investigate the effect of NS398

cyclooxygenase-2（COX-2）-inhibitor

on the antergy of lysophosphatidic acid （LPA）for the inhibiting effect of cisplatin（DDP）in the proliferation of huanman ovarian cancer

and to analyze its possible mechanism. Methods:Human ovarian cancer cell line（3AO）were cultured in vitro.The effect of NS398 on the antergy of LPA for the inhibiting effect of DDP in the proliferation of 3AO cells were measured by MTT.Cell cycle of 3AO were analysed by flow cytometry（FCM）.The effect of LPA alone or in combination with NS398 on the expression of COX-2 of 3AO cells were measured with RT-PCR.Results:LPA showed antergy on the inhibiting effect of DDP in the proliferation of 3AO cells

and decrease the proportion of cells in the G

phase of the cell cycle.Whereas

combined with NS398 in culture

the proliferation of were significantly inhibited

and the the proportion of cells in the G

phase of the cell cycle was raised.RT-PCR results revealed that 40μmol/L LPA could promote the expression of COX-2 in 3AO cells

and in combination with NS398

the expression of COX-2 was decreased.Conclusion:The reverse effect of NS398 for the antergy of LPA on the inhibiting effect of DDP in the proliferation of 3AO cells may be completed by inhibiting the expression of COX-2 in these cells.

关键词

溶血磷脂酸环氧化酶-2顺铂NS3983AO细胞卵巢癌

Keywords

Lysophosphatidic acidCyclooxygenase-2cisplatinNS3983AO cellsovarian cancer

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